rss_2.0Acta Chimica Slovaca FeedSciendo RSS Feed for Acta Chimica Slovacahttps://sciendo.com/journal/ACShttps://www.sciendo.comActa Chimica Slovaca Feedhttps://sciendo-parsed.s3.eu-central-1.amazonaws.com/6470798971e4585e08a9dff6/cover-image.jpghttps://sciendo.com/journal/ACS140216Theoretical investigation of aniline derivatives: Correlation of theoretical reaction Gibbs free energies with experimental oxidation potentialshttps://sciendo.com/article/10.2478/acs-2024-0010<abstract><title style='display:none'>Abstract</title> <p>Anilines and their derivatives are used in industrial production of dyes, pharmaceuticals, plastics, and synthetic antioxidants. Abstraction of electron and formation of a cation radical represent a significant step in the reactivity of this group of substances. The aim of this study was to theoretically investigate the effect of a substituent on the oxidative electrochemical potential of 64 species of aniline derivatives. Quantum-chemical calculations were performed using the composite G4 method. The obtained linear dependences for reaction Gibbs free energies were correlated with the Hammett constants and available experimental values.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/acs-2024-00102024-12-18T00:00:00.000+00:00Theoretical and experimental study of monoethanolamides and diethanolamides as potential additives to fossil diesel fuelhttps://sciendo.com/article/10.2478/acs-2024-0011<abstract><title style='display:none'>Abstract</title> <p>In this work, theoretical and experimental study of monoethanolamides (MEAD) and diethanolamides (DEAD) is presented. The aim was to investigate the thermodynamics of addition reactions used in the synthesis of MEAD and DEAD. The reactions have exothermic character and alkyl elongation affects the reaction enthalpy of DEAD production reactions. In the experimental part, the prepared MEAD and DEAD samples were preliminary tested as a potential additive in diesel fuel. The research focused on measuring three key properties: i.e., foaming, lubricity, and cetane number. The results suggest that MEAD is a suitable additive for diesel fuel.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/acs-2024-00112024-12-18T00:00:00.000+00:00On the nature of copper binding to benzenehttps://sciendo.com/article/10.2478/acs-2024-0009<abstract> <title style='display:none'>Abstract</title> <p>Adsorption of copper atom on benzene surface has been studied at the <italic>ab initio</italic> MP2 and CCSD(T) theory levels. CCSD(T)/CBS binding energies of the copper atom adsorbed on hollow, top, and bridge positions are 16.77, 19.27 and 21.08 kJ/mol, respectively. Adsorption at the bridge position represents the most stable structure of the Cu-benzene complex with charge transfer from benzene to the copper atom playing a key role.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/acs-2024-00092024-12-18T00:00:00.000+00:00Reducing the impact of artificial blue light on the skin: A spectroscopic studyhttps://sciendo.com/article/10.2478/acs-2024-0007<abstract> <title style='display:none'>Abstract</title> <p>As people spend many hours looking at digital screens, the negative effects of artificial blue light are becoming more apparent. While most research has focused on its effects on eyes, less is known about the effects of blue light on the skin, where similar photoreceptors are located. Unlike the sunscreens against UVB and UVA radiation, there is no standard method for determining skin protection against blue light. The lipophilic complex Carotolino, a system combining carrot root extract, carrot seed oil, and β-carotene, was chosen as a model substance for this research. Spectrophotometric investigation demonstrated the ability of Carotolino to absorb radiation in the blue light region (400—500 nm). After a 60-minute LED@450 nm exposure, corresponding to the maximum wavelength of radiation from the displays of common smartphones, only small changes (1.4 %) in the optical spectra were observed. The spectra showed sufficient photostability of Carotolino and its stabilizing effect on the photolabile Ubiquinone. In the 415—455 nm wavelength range associated with oxidative stress, Carotolino (0.4 % wt.) reduced blue light by ∼97.0 %. EPR spin trapping showed that blue light with a maximum wavelength of 450 nm causes significant formation of reactive free radicals, which can be partially eliminated by the application of Carotolino. The results confirmed the suitability of both methods to evaluate the effectiveness of substances to reduce physical impact of incident light on the skin. Further methods are needed to investigate biological protection of skin against blue light by promising substances.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/acs-2024-00072024-11-17T00:00:00.000+00:00Advancements and challenges in extraction and determination of pesticide residues in soya productshttps://sciendo.com/article/10.2478/acs-2024-0006<abstract> <title style='display:none'>Abstract</title> <p>Soya beans, a vital source of protein, oil, and dietary fibre, are cultivated extensively, with a growing demand since 1950. However, the extensive use of pesticides in soya bean cultivation poses potential risks to human health and the environment, especially given the lack of strict regulations for maximum residue levels in soya-based products within the European Union. This study provides a comprehensive overview of isolation and determination methods of pesticide residues from soya products, emphasising the challenges posed by the high-fat content of soya beans. Various chromatographic techniques coupled with mass spectrometry are discussed for their efficacy in pesticide residues analysis. The manuscript also reviews sample preparation techniques, with a focus on the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method, and its modifications to enhance extraction and purification efficiency. Additionally, the study explores the application of liquid-liquid extraction and solid-phase extraction in the analysis of soya oil and beverages and innovative methods, such as dispersive liquid-liquid microextraction and matrix solid-phase dispersion, for comprehensive pesticide residue analysis. This review highlights the importance of multiresidue analytical methods in ensuring the safety of soya products thereby supporting regulatory compliance and public health protection.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/acs-2024-00062024-11-17T00:00:00.000+00:00Quantum-chemical studies of infrared spectra of N labeled diazene isomershttps://sciendo.com/article/10.2478/acs-2024-0008<abstract> <title style='display:none'>Abstract</title> <p>The geometry of <italic>trans</italic>-HN=NH, <italic>cis</italic>-HN=NH and N=NH<sub>2</sub> containing no, one or two <sup>15</sup>N labeled atoms was optimized. The corresponding infrared vibrations were evaluated using a linear scaling factor. For each of these compounds at least one vibration can be found, which enables to distinguish between heteroisotopic <sup>14</sup>N=<sup>15</sup>N and homoisotopic <sup>14</sup>N=<sup>14</sup>N or <sup>15</sup>N=<sup>15</sup>N species. Independent of the <sup>15</sup>N labeling, only <italic>trans</italic>-conformation should be found in the reaction mixture under equilibrium conditions.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/acs-2024-00082024-11-17T00:00:00.000+00:00Prediction of selected properties of aflatoxin molecules by the QSAR methodhttps://sciendo.com/article/10.2478/acs-2024-0004<abstract> <title style='display:none'>Abstract</title> <p>Aflatoxins are naturally occurring compounds produced by fungi, mainly of <italic>Aspergillus</italic> species. All aflatoxins are proved to cause acute toxicity to human health, some even causing chronic diseases such as cancer. These molecules clearly proved that even natural molecules can be directly related to cancer and the formation of tumours. In general, aflatoxins can be characterised as organic compounds; B1, G1, B2, and G2 are produced directly by fungi and these four are subsequently metabolised in biological systems of microbes, animals or humans into other forms, such as M1, EB1, AFL etc. This manuscript provides a brief overview of 14 aflatoxins, their molecular structure and its possible relationship to aflatoxins biological activity. This information, in combination with additional calculations, offers the possibility to investigate the mentioned compounds and their properties using the QSAR approach.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/acs-2024-00042024-08-15T00:00:00.000+00:00Novel synthesis of known carcinogen precursorshttps://sciendo.com/article/10.2478/acs-2024-0005<abstract> <title style='display:none'>Abstract</title> <p>5-Amino-1-methylbenzimidazole (<bold>2)</bold> prepared from 2,4-dinitrochlorobenzene in four steps is a suitable reagent for nucleophilic vinylic substitution with various enolethers (<bold>3)</bold> thus affording <italic>N</italic>-substituted enaminoesters — aminoethylene derivatives (<bold>4)</bold>. Their thermal cyclocondensation reaction under the Gould-Jacobs protocol gives regioselectively angularly annelated 8-substituted imidazo[4,5-<italic>f</italic>]quinolones (<bold>5)</bold>, whose mechanism of origin has been attempted. The obtained results give suitable structures for the synthesis of food-borne carcinogen IQ (<bold>1</bold>).</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/acs-2024-00052024-08-15T00:00:00.000+00:00Solvent retention capacity as a useful tool for quality evaluation of flours produced from Slovak bred wheat varietieshttps://sciendo.com/article/10.2478/acs-2024-0003<abstract> <title style='display:none'>Abstract</title> <p>Wheat varieties (IS Danubius and MS Luneta) bred in Slovakia were assessed for their bakery potential. It was found that flour produced from IS Danubius was characterised by significantly higher level of wet and dry gluten content (30.91 and 20.53 %, respectively) and exhibited higher gluten swelling capacity (29.3 %) in comparison to commercial wheat flour that is usually available in Slovak markets. Solvent retention capacity (solvation in specific solvents) of the tested flours was also determined. The measurements showed that these parameters significantly differ from those determined for commercially available flour, whereas the flour produced from IS Danubius wheat variety had the highest lactic acid retention capacity (124.15 %). Correlation analysis indicated strong positive correlations between solvent retention capacity values and gluten characteristics. Furthermore, it was found that baked rolls prepared from IS Danubius flour showed significantly higher loaf volume compared to commercial wheat flour. This study proves lactic acid retention capacity and sucrose retention capacity as parameters enabling the prediction of gluten network quality in dough and of qualitative parameters of baked goods. From the sensory evaluation resulted that the highest score for overall acceptance was observed in IS Danubius baked rolls, which significantly differs from both MS Luneta and commercial wheat flour.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/acs-2024-00032024-04-06T00:00:00.000+00:00Substitution effect of phenol derivatives on electrochemical oxidation potentials: Correlation of theoretical reaction Gibbs free energieshttps://sciendo.com/article/10.2478/acs-2024-0002<abstract> <title style='display:none'>Abstract</title> <p>Reaction Gibbs free energies for electron abstraction from phenol and its 74 derivatives were calculated using the composite <italic>ab-initio</italic> approach (G4) in combination with the implicit solvation model. Resulting values were correlated with 38 oxidation potentials obtained from cyclic voltammetry measurements. Substitution effect and the role of substituents in <italic>ortho</italic>-, <italic>meta</italic>-, and <italic>para</italic>-position were also quantified by Hammett constants. The evaluated linear dependences can be used to reliably estimate electrochemical potentials of substituted phenols solvated in water.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/acs-2024-00022024-02-12T00:00:00.000+00:00Atomic partial charge model in chemistry: chemical accuracy of theoretical approaches for diatomic moleculeshttps://sciendo.com/article/10.2478/acs-2024-0001<abstract> <title style='display:none'>Abstract</title> <p>Atomic partial charges cannot be physically measured but they play a significant role in many chemical theories and theoretical models. Therefore, they are, evaluated from experimentally acquired properties or calculated by quantum chemistry computational methods. This study is focused on determining chemical accuracy of various theoretical methods of computing atomic partial charges based on quantum chemistry. Values of gas-phase atomic partial charges were acquired by Mulliken (MUL) population analysis, natural bond analysis (NBO), Merz-Singh-Kollman (MSK) scheme, and atomic polar tensor (APT) charges computed considering Density Functional Theory and <italic>ab initio</italic> Møller-Plesset up to the second order levels. Correlations between the calculated values were determined by principal component analysis (PCA) and further confirmed by linear regression. The best agreement between experimentally evaluated atomic partial charges and theoretical values was obtained with the MSK scheme.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/acs-2024-00012024-01-20T00:00:00.000+00:00Development and validation of HPLC-FLD method for aflatoxin M determination in milk and dairy productshttps://sciendo.com/article/10.2478/acs-2023-0010<abstract> <title style='display:none'>Abstract</title> <p>Milk and dairy products are the most consumed foods in human diet and their safety is in the attention centre of control authorities. Aflatoxin M<sub>1</sub> (AFM<sub>1</sub>) is a dangerous toxin that can occur in milk and dairy products as a metabolite formed from aflatoxin B<sub>1</sub> contained in contaminated animal feed. Therefore, the aim of this study was to develop and validate a reliable method for the determination of AFM<sub>1</sub> content in milk and dairy products based on HPLC with fluorescence detection employing immunoaffinity columns (IAC) pre-treatment. Optimal chromatographic separation of AFM<sub>1</sub> was achieved using a water/acetonitrile mixture (80/20, v/v) as a mobile phase, column with C<sub>18</sub> stationary phase maintained at 25 °C, and fluorescence detection at excitation wavelengths of 360 nm and emission of 440 nm. Efficacy of AFM<sub>1</sub> extraction from the samples was found to be influenced by the elution agent composition. The best results were obtained using 1.25 mL of acetonitrile/methanol (3/2, v/v) and 1.25 mL of water. Validation parameters of the proposed method met the criteria set by the European legislation with the limits of detection and quantification at 0.002 and 0.007 µg/kg, respectively. Also, suitability of the method was confirmed by its application for AFM<sub>1</sub> determination in certified reference material. Finally, the method was applied for AFM<sub>1</sub> determination in 25 milk and dairy products collected in Slovakia; the AFM<sub>1</sub> content was below the limit of quantification. It was concluded that the method is suitable for AFM<sub>1</sub> content monitoring in milk and dairy products.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/acs-2023-00102023-12-15T00:00:00.000+00:00Two novel potent perfluorophenylhydrazone derivatives, 1-((4-bromothiophen-2-yl)methylene)-2-(perfluorophenyl) hydrazine, and 1-((4-bromo-5-methylthiophen-2-yl)methylene)-2-(perfluorophenyl)hydrazine as multi-target compounds to combat Alzheimer disease and their crystal, molecular, and electronic propertieshttps://sciendo.com/article/10.2478/acs-2023-0008<abstract> <title style='display:none'>Abstract</title> <p>Two potent novel perfluorophenylhydrazone derivatives are presented as multi-target compounds to combat Alzheimer disease C<sub>11</sub>H<sub>4</sub>BrF<sub>5</sub>N<sub>2</sub>S, 1-((4-bromothiophen-2-yl)methylene)-2-(perfluorophenyl) hydrazine, (I) and C<sub>12</sub>H<sub>6</sub>BrF<sub>5</sub>N<sub>2</sub>S, 1-((4-bromo-5-methylthiophen-2-yl)methylene)-2-(perfluorophenyl) hydrazine, (II), which can potentially be improved by further design. Their multi-target structures and features have been combined as potential AD therapeutics. Crystals (I), and (II), are molecules with two rings and a hydrazone part as a centre of the molecule. The compounds have been synthesised and characterised by elemental spectroscopic (<sup>1</sup>H-NMR) analysis. Crystal structures of the solid phase were determined by single crystal X-ray diffraction method. Both compounds crystallise in the monoclinic space group with <italic>Z</italic> = 4 and <italic>Z</italic> = 2 molecules per unit-cell. Compound (I) crystallises as a racemate in the centrosymmetric space group and compound (II) crystallises as a non-racemate in the non-centrosymmetric space group. Their “absolute configuration and conformation for bond values” were derived from the anomalous dispersion (rmad) for (II). Crystal structures revealed diverse non-covalent interactions such as intra- and inter-hydrogen bonding, π-ring…<italic>π</italic>-ring, C—H…<italic>π</italic>-ring. The expected stereochemistry of hydrazones atoms C7, N2 and N1 were confirmed for (I) and (II). Both molecules show “boat conformation” like a 6-membered ring. Results of single crystal studies were reproduced with the help of Hirshfeld surface study and Gaussian software.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/acs-2023-00082023-12-15T00:00:00.000+00:00Extraction of biologically active compounds from : Comparison of techniques and multiple response optimizationhttps://sciendo.com/article/10.2478/acs-2023-0009<abstract> <title style='display:none'>Abstract</title> <p>The presented paper deals with the selection of most efficient extraction method for obtaining biologically active compounds (mainly antioxidants) from the black chokeberry (<italic>Aronia melanocarpa</italic>) fruits. Two conventional methods (maceration and Soxhlet extraction) as well as a more recent accelerated solvent extraction (ASE) were employed. The extracts were evaluated by means of their overall yield, antioxidant activity (TEAC assay), total phenolic content (TPC), ascorbic acid (AA), and malic acid (MA) content. Both Soxhlet extraction and ASE led to the highest overall yields (42 %) of the extract; however, in terms of TEAC and TPC, the extracts obtained by ASE were usually superior. The highest TEAC value (227.7 mg TE/g) as well as the highest TPC (67.9 mg GAE/g) were obtained by ASE using 40 % (v/v) ethanol at 140 °C after 30 min. The ASE method was further analyzed using a 2<sup>3</sup>-factorial design where the effect of temperature (40—140 °C), extraction time (5—30 min), and solvent composition (40—96 % ethanol) was investigated. While temperature and solvent composition significantly affected the extract properties, the effect of extraction time was small or even insignificant. The regression model obtained from ANOVA was further used for multiresponse optimization of ASE conditions using the global desirability function as a criterion for overall extract quality.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/acs-2023-00092023-12-15T00:00:00.000+00:00Comparison of gut resistomes in healthy individuals and patients with severe alcoholic hepatitishttps://sciendo.com/article/10.2478/acs-2023-0011<abstract> <title style='display:none'>Abstract</title> <p>Human gut microbiota has been in the centre of scientific interest for a long period of time. Overall health status of an individual has a great impact on the composition of gut microbiota; however, gut microbiota can affect human health. Antibiotic resistance genes (ARGs) are often a part of human gut microbiome. In this paper, total genomic DNA was extracted from stool samples of 147 healthy individuals and of 45 patients with severe alcoholic hepatitis. The presence of six common ARGs (<italic>bla</italic><sub>TEM</sub>, <italic>bla</italic><sub>SHV</sub>, <italic>bla</italic><sub>OXA</sub>, <italic>vanA, tet</italic>(A), <italic>tet</italic>(E)) was analysed in the genomic DNA by end-point PCR. The results show significantly higher occurrence of ARGs in the DNA samples from patients (<italic>p</italic> = 0.0001) showing multiple ARGs significantly more often than in healthy individuals (<italic>p</italic> = 0.00003). Antibiotic treatment in patients strongly correlated with the occurrence of ARGs (<italic>p</italic> = 0.0038). Nutrition and sex of healthy individuals did not have significant effect on the occurrence of ARGs (<italic>p</italic> = 0.156; <italic>p</italic> = 0.456). ARGs’ occurrence in healthy individuals was the highest in the oldest age group, but the age of individuals and ARGs’ occurrence were not related (<italic>p</italic> = 0.617). In conclusion, the results underline the importance of health for normal functioning of gut microbiota. Antibiotic resistance represents a challenge in the treatment of patients with liver diseases.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/acs-2023-00112023-12-15T00:00:00.000+00:00Extraction of bioactive coumarins from lime peel as sample pretreatment before chromatographic analysishttps://sciendo.com/article/10.2478/acs-2023-0007<abstract> <title style='display:none'>Abstract</title> <p>Analysis of lime peel was applied to assess their suitability for various intended purposes, e.g., application in perfumery, cosmetics, and cleaning products, or as a source of bioactive or other value-added compounds. Targeted analysis allows wider usability of the waste part of these natural materials. In the present study, a novel, efficient, lab-simple, time-saving analytical method for coumarins determination in lime peel, including sample pretreatment by extraction and quantification by HPLC with fluorescence detection (FLD), is introduced. Optimal conditions of ultrasound assisted extraction included water as extraction solvent, temperature of 40 °C, and extraction time of 10 min. A magnetic molecularly imprinted polymer was employed as solid phase extraction adsorbent for primary extract cleaning, isolation, and enrichment of coumarins before chromatographic analysis. Recovery of herniarin and umbelliferone was more than 86 % ((RSD ≤ 4.8 %). Linear range of 50—1000  ng/mL with correlation coefficient above 0.998 was obtained for the proposed HPLC-FLD method. The limit of quantification was 8.2 and 44 ng/mL for herniarin and umbelliferone, respectively. These results show that the proposed sample pretreatment procedure is suitable for analytical purposes and is perspective also for the analysis of other citrus samples, as well as for future scale-up preparative isolation of bioactive coumarins from citrus peel.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/acs-2023-00072023-09-30T00:00:00.000+00:00Utilization of as an alternative ingredient in value added bread and pasta productshttps://sciendo.com/article/10.2478/acs-2023-0005<abstract><title style='display:none'>Abstract</title> <p>Natural sources and healthy cereal-based food have recently received a lot of attention by both professionals and the common population for improving overall well-being. In this case, <italic>Opuntia</italic> cactus and <italic>Opuntia</italic> derivates show high potential in the production of health-promoting cereal-based products.</p> <p>This study comprehensively reviews nutritional composition, health benefits of <italic>Opuntia</italic> spp. and its utilisation in the production of bread and pasta products. Moreover, the effect of this ingredient on the rheological properties of wheat dough and physical parameters as well as sensory properties of incorporated products is also discussed.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/acs-2023-00052023-08-29T00:00:00.000+00:00Colour masterbatches and their use in polylactic acid fibres dyeinghttps://sciendo.com/article/10.2478/acs-2023-0006<abstract><title style='display:none'>Abstract</title> <p>This article is focused on the preparation of colour masterbatches from renewable sources based on polylactic acid, which were modified with inorganic pigments. Titanium dioxide, carbon black, and iron oxides were used as inorganic pigments. Due to the composition of the prepared colour PLA masterbatches, the influence of the inorganic pigment on the rheological and thermal properties was monitored. Rheological properties were determined using a capillary viscosimeter and a rotary rheoviscosimeter with a geometry plate/plate. The colour PLA masterbatches prepared in this way were subsequently used to dye polylactic acid fibres in the mass. The prepared fibres were drawn to the maximum drawn ratio <italic>λ</italic><italic><sub>max</sub></italic>. In case of fibres, the influence of composition and drawn ratio on thermal and mechanical properties was monitored.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/acs-2023-00062023-08-29T00:00:00.000+00:00Stability of ferrate during long-term storagehttps://sciendo.com/article/10.2478/acs-2023-0004<abstract> <title style='display:none'>Abstract</title> <p>Surface water and groundwater are polluted with pharmaceuticals, detergents, pesticides, and many other substances. Application of ferrates seems to be a perspective option for wastewater treatment as ferrates are not only powerful oxidizing agents but also an excellent disinfecting and coagulating agents decomposing many stable inorganic, organic, and biological compounds. Final products of their decomposition do not include carcinogenic or toxic products. In this paper, stability of electrochemically prepared potassium ferrate encapsulated in packaging materials printed on a 3D printer was monitored. In the experiment, electrochemically prepared potassium ferrate with different purity (21.4 %, 63.5 % and 67.3 %) was used. Stability of potassium ferrate was monitored for one month and that of other ferrates for three months. Different storage conditions of ferrate samples were also compared. Storage conditions had a significant influence on the ferrate stability.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/acs-2023-00042023-06-09T00:00:00.000+00:00Study on secondary metabolites of F742 and their role in antibiosishttps://sciendo.com/article/10.2478/acs-2023-0002<abstract> <title style='display:none'>Abstract</title> <p>Soil-borne pathogenic fungi responsible for serious damage in agriculture are widely distributed. Traditional approach to control these pathogens leads to the use of chemical fungicides, many of which have proven harmful side effects on the environment. Mycoparasitic fungi of the genus <italic>Trichoderma</italic> have been used for pathogens biocontrol as well as for their ability to promote plant growth. As increased mycoparasitic activity of mutant strain <italic>Trichoderma atroviride</italic> F742 has been observed, its use as a biocontrol agent might be considered. In this work, we focused on mycoparasitic activity of the strain <italic>T. atroviride</italic> F742 prepared by UV mutagenesis (from parental strain <italic>T. atroviride</italic> F534) and the physiological role of <italic>T. atroviride</italic> metabolites in antibiosis.</p> <p><italic>T. atroviride</italic> F742 shows remarkable mycoparasitic activity attacking and colonizing phytopathogens <italic>(Alternaria alternata</italic>, <italic>Botrytis cinerea</italic>, <italic>Fusarium culmorum</italic>). Its isolated metabolites inhibit the growth of bacteria, yeasts, and fungi <italic>Candida albicans, A. alternata</italic> and <italic>F. culmorum.</italic> As different expression patterns in the secondary metabolites production of ABC transporters have been observed, we suggest their role in transport of secondary metabolites produced by <italic>T. atroviride</italic> F742.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/acs-2023-00022023-06-09T00:00:00.000+00:00en-us-1