rss_2.0Revista Romana de Medicina de Laborator FeedSciendo RSS Feed for Revista Romana de Medicina de Laboratorhttps://sciendo.com/journal/RRLMhttps://www.sciendo.comRevista Romana de Medicina de Laborator Feedhttps://sciendo-parsed.s3.eu-central-1.amazonaws.com/64736bbb4e662f30ba53d7ea/cover-image.jpghttps://sciendo.com/journal/RRLM140216Monocyte to high-density lipoprotein cholesterol ratio is correlated with baseline anthropometric measurements in patients with obesity but not with weight-loss process after sleeve gastrectomy - An observational cohort studyhttps://sciendo.com/article/10.2478/rrlm-2024-0002<abstract> <title style='display:none'>Abstract</title> <p><bold>Introductionː</bold> A strong connection between inflammation and obesity was repeatedly described, with the latter defined as a chronic low-degree systemic inflammatory state. This study analyzed the correlations between inflammatory blood indexes and both baseline anthropometric measurements and the weight–loss process after bariatric surgery.</p> <p><bold>Methodsː</bold> An observational study was conducted on patients with obesity admitted for metabolic surgery in a private and a public hospital. The primary endpoints were to establish correlations between baseline inflammatory ratios i.e. neutrophil to lymphocyte (NLR), platelet to lymphocyte (PLR), monocyte to lymphocyte (MLR), monocyte to high-density lipoprotein cholesterol (MHR) ratios, systemic inflammatory index (SII) and anthropometric measurements. Secondary endpoints were to find out if these ratios measured at baseline are predictive factors for weight loss after bariatric surgery.</p> <p><bold>Results</bold>: In the present study, we included 191 patients, mean age 39.1±10,7 and mean BMI 42,2±6,5 kg/m2. There was a positive correlation between MHR and all anthropometric measurements taken at baseline; a direct correlation was also found for MLR in regards to initial weight and waist circumference. No statistical correlations were found between the above-mentioned indexes and the weight loss process (measured as a percentage of excess weight loss - %EWL) at different follow-up timeframes.</p> <p><bold>Conclusionsː</bold> Monocyte to high-density lipoprotein cholesterol ratio (MHR) was positively associated with baseline anthropo-metric measurements in patients with obesity. The above-mentioned inflammatory ratios did not correlate with the weight loss process after bariatric surgery, thus they should not be used as predictors of good postoperative results.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/rrlm-2024-00022024-01-29T00:00:00.000+00:00Value of four-dimensional computed tomography angiography combined with stromal cell-derived factor-1 for differentiating ruptured intracranial aneurysms and assessing risk of ruptureshttps://sciendo.com/article/10.2478/rrlm-2024-0001<abstract> <title style='display:none'>Abstract</title> <p>Background: To analyze the value of four-dimensional computed tomography angiography (4D-CTA) combined with stromal cell-derived factor-1 (SDF-1) for differentiating ruptured intracranial aneurysms and assessing the risk of ruptures.</p> <p>Methods: Fifty patients with unruptured intracranial aneurysms and 50 patients with ruptured ones were included in non-rupture group 1 and rupture group 1, respectively. All patients underwent 4D-CTA and 3D-CTA, and the serum SDF-1 level was detected. Non-rupture group 1 was followed up for 12 months. On this basis, the patients with ruptured aneurysms were included in rupture group 2 and those without ruptured aneurysms were assigned to non-rupture group 2.</p> <p>Results: The AUC values of Wn, AR, L, SR, SDF-1 and their combination for the diagnosis of ruptured intracranial aneurysms were all &gt;0.70, especially their combination. Wn, AR, L, and SR were higher in rupture group 2 than those in non-rupture group 2 (P&lt;0.05). The level of SDF-1 in rupture group 2 [(142.48±11.23) μg/L] was higher than that in non-rupture group 2 [(128.03±10.28) μg/L] (P&lt;0.05). Wn, AR, L, SR, and SDF-1 and their combination all had the AUC values of &gt;0.70 for the prediction of ruptured intracranial aneurysms, especially their combination. The CT values of the internal carotid artery and middle cerebral artery and the CT value and noise of brain parenchyma in 4D-CTA were higher than those in 3D-CTA (P&lt;0.05).</p> <p>Conclusions: 4D-CTA combined with SDF-1 can effectively differentiate ruptured intracranial aneurysms and predict the risk of ruptures.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/rrlm-2024-00012024-01-29T00:00:00.000+00:00Joint EFLM-COLABIOCLI recommendation for venous blood sampling - v 1.1, June 2018https://sciendo.com/article/10.2478/rrlm-2024-0004<abstract> <title style='display:none'>Abstract</title> <p>Acest document oferă o recomandare comună a Federației Europene de Chimie Clinică și Medicină de Laborator (EFLM), Grupului de lucru pentru faza preanalitică (WG-PRE) și Grupului de lucru din America Latină pentru Faza preanalitică (WG-PRE-LATAM) al Confederației Americii Latine de Biochimie Clinică (COLABIOCLI) pentru recoltarea sângelui venos. Documentul oferă îndrumări asupra cerințelor pentru asigurarea faptului că procedura de recoltare a sângelui este una sigură, centrată pe pacient și oferă îndrumări practice despre cum să fie depășite cu succes potențiale bariere și obstacole în calea difuzării și implementării ei. Publicul țintă pentru această recomandare este personalul medical implicat direct în procesul de recoltare a sângelui. Această recomandare se aplică în cazul utilizării unui sistem închis de recoltare a sângelui și nu oferă recomandări pentru recoltarea sângelui cu seringi și catetere în sistem deschis. Mai mult, acest document nu abordează obținerea consimțământului pacientului, solicitarea testelor, manipularea și transportul probelor și nici recoltarea de la copii și pacienții inconștienți. Procedura recomandată se bazează pe cele mai bune dovezi disponibile. Fiecare pas a fost evaluat folosind un sistem care punctează calitatea dovezilor și puterea recomandării. Procesul de evaluare a fost realizat la mai multe întâlniri față în față implicând aceleași părți interesate menționate anterior. Principalele părți ale acestei recomandări sunt: 1) Proceduri de pre-recoltare, 2) Procedura de recoltare, 3) Proceduri de post-recoltare şi 4) Implementarea. O primă schiță a recomandării a fost transmisă membrilor EFLM pentru consultare publică. A fost invitat și WG-PRE-LATAM pentru a comenta documentul. O versiune revizuită a fost trimisă spre vot tuturor membrilor EFLM și COLABIOCLI și a fost aprobată oficial de 33 dintre cei 40 de membri EFLM și toți membri COLABIOCLI. Încurajăm profesioniștii din toată Europa şi America Latină să adopte şi să implementeze această recomandare pentru a îmbunătăți calitatea practicilor de recoltare a sângelui și creșterea siguranței pacientului și personalului medical.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/rrlm-2024-00042024-01-29T00:00:00.000+00:00Anti-thyroid peroxidase (TPO) antibodies – Comparative analysis of two automatic methods, ECLIA and CMIAhttps://sciendo.com/article/10.2478/rrlm-2024-0009<abstract> <title style='display:none'>Abstract</title> <p><bold>Introduction</bold>: Anti-thyroid peroxidase autoantibodies (TPO) is an essential diagnostic tool for autoimmune disorders of the thyroid gland. However, TPO results are not always comparable due to differences between methods. Here, we aimed to investigate the differences between two modern laboratory methods for TPO measurement: electrochemiluminescence (ECLIA) and chemiluminescence microparticle (CMIA) immunoassays.</p> <p><bold>Methods</bold>: A total of 234 serum samples were tested on two methods: Cobas-e601 (ECLIA) and Alinity i (CMIA). TPO results were compared statistically both quantitatively and qualitatively (results were coded as positive/negative, according to ECLIA/CMIA reference ranges.</p> <p><bold>Results</bold>: The precisions of both methods were acceptable compared with the claims of the manufacturer. There was a very strong, but unsatisfactory correlation between the two methods (Pearson r=0.85). Passing-Bablok regression revealed a significant deviation from linearity (Cusum p&lt;0.01) and an unacceptable quantitative relationship: intercept −7.61, slope 1.10. Moreover, a visual analysis of overall and medical decision level-focused Bland-Altman plots confirmed the lack of quantitative agreement. As for the qualitative analysis, the concordance rate between methods was 218/234 (93.1%). The agreement was considered good to very good according to the inter-rater agreement test: weighted Cohen κ = 0.805.</p> <p><bold>Conclusions</bold>: The qualitative agreement between Cobas-e601 (ECLIA) and Alinity i (CMIA) was good, therefore the two methods may be used indiscriminately for initial testing of patients suspected of thyroid gland autoimmune diseases. However, due to poor quantitative agreement, the two methods should not be used interchangeably for monitoring as the results may mislead both physicians and patients, possibly leading to medical errors.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/rrlm-2024-00092024-01-29T00:00:00.000+00:00Identification of shared hub genes in lung cancer and rheumatoid arthritis patients using bioinformatics approacheshttps://sciendo.com/article/10.2478/rrlm-2024-0007<abstract> <title style='display:none'>Abstract</title> <p><bold>Objectives</bold>:To identify key genes common to lung cancer and rheumatoid arthritis through WGCNA co-expression network and MCC algorithm analysis.</p> <p><bold>Methods</bold>: Initially, chip data related to lung cancer and rheumatoid arthritis were obtained from the GEO database for data integration and differential analysis, leading to the identification of key differentially expressed genes. Subsequently, WGCNA was utilized to construct a co-expression network, identifying susceptible modules and core genes. Further, common core genes in lung cancer and rheumatoid arthritis were identified through Venn diagrams, assessing their diagnostic accuracy in disease, analyzing differential expression, and constructing a co-expression network. Finally, GO and KEGG enrichment analyses were conducted to understand the functions and pathway enrichment of these core genes, and potential target drugs were predicted.</p> <p><bold>Results</bold>: Six lung cancer-related and three rheumatoid arthritis-related gene co-expression modules were constructed using WGCNA. The Turquoise module was identified as the susceptible module for lung cancer, while the Blue module was for rheumatoid arthritis. A total of 953 genes were included in the lung cancer hub genes, and 152 in the rheumatoid arthritis hub genes. Finally, 92 potential target drugs were predicted through the DGIdb database that may regulate the expression of 11 common hub genes.</p> <p><bold>Conclusion</bold>: We identified 24 common hub genes for lung cancer and rheumatoid arthritis, with the top 6 ranked by the MCC algorithm being FGR, SLA, GZMH, CSF2RB, PRF1, and CCRL2. This study paves the way for further exploration of the common pathogenesis of lung cancer and rheumatoid arthritis. However, further in vivo and in vitro experiments are required for validation and support.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/rrlm-2024-00072024-01-29T00:00:00.000+00:00Predictive value of expression of the CXCR4/Akt signaling pathway in the peripheral blood for brain gliomahttps://sciendo.com/article/10.2478/rrlm-2024-0008<abstract> <title style='display:none'>Abstract</title> <p><bold>Background</bold>: The aim of this study was to investigate the activation of the C-X-C chemokine receptor type 4 (CXCR4)/Akt signaling pathway in the peripheral blood of individuals diagnosed with brain glioma and assess its potential as a predictive marker.</p> <p><bold>Methods</bold>: A study was carried out on 120 patients diagnosed with brain glioma who were admitted between September 2015 and October 2016 (referred to as the brain glioma group). Additionally, a control group consisting of 100 healthy individuals who underwent physical examinations during the same time frame was included. According to the postoperative follow-up results, the patients with brain glioma were divided into death subgroup (n=30) and survival subgroup (n=85). Reverse transcription-polymerase chain reaction was used to identify the presence of molecules associated with the CXCR4/Akt signaling pathway in the peripheral blood samples.</p> <p><bold>Results</bold>: The expressions of the CXCR4/Akt signaling pathway-related molecules differed significantly among tumor World Health Organization (WHO) grades and clinical outcome subgroups (P&lt;0.05). The CXCR4 expression showed a significant correlation with the WHO grade of the tumor, Akt mRNA, E-cadherin, N-cadherin, and vimentin (P&lt;0.05). The prognosis was significantly influenced by the elevated levels of CXCR4/Akt, tumor WHO grade, E-cadherin, N-cadherin, and vimentin, which acted as autonomous risk factors. The prognostic prediction accuracy of CXCR4/Akt was 90.48% for sensitivity and 94.87% for specificity, with a significant area under the receiver operating characteristic curve of 0.908 (P&lt;0.05). Patients with high levels of CXCR4/Akt signaling pathway-related molecules had a considerably lower 5-year survival rate compared to those with low levels (21.08% vs. 46.37%, P&lt;0.05).</p> <p><bold>Conclusions</bold>: The expression of the CXCR4/Akt signaling pathway is significantly up-regulated in the peripheral blood of patients with brain glioma and is closely related to malignant tumor transformation.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/rrlm-2024-00082024-01-29T00:00:00.000+00:00Function of the S1P pathway in hypoxia-induced cardiovascular failurehttps://sciendo.com/article/10.2478/rrlm-2024-0006<abstract> <title style='display:none'>Abstract</title> <p><bold>Background</bold>: Vascular failure (VF) and heart failure (HF) are extremely harmful and are the primary causes of hypoxia. Our previous results have shown that the sphingosine-1-phosphate (S1P) pathway was involved in regulating intermittent hypoxia–induced vascular defection, but the clinical role and molecular mechanism of the S1P pathway remain unclear.</p> <p><bold>Methods</bold>: Normalized relative expression values and differentially expressed genes were downloaded in GSE145221 from the Gene Expression Omnibus dataset. WGCNA was used to construct a gene co-expression network. The Spearman correlation matrix was used to identify the top 500 highly correlated genes with the S1P pathway genes. R package clusterProfiler was used to perform Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses on the WGCNA modules. Homer software was utilized to identify regulatory motifs in the promoter and gene body regions of S1P pathway genes. An intermittent hypoxic injury cell model was induced by chronic intermittent hypoxia (CIH). ROS and TUNEL staining and Western blot were used to detect cell apoptosis and reactive oxygen species.</p> <p><bold>Results</bold>: The transcriptional regulatory regions of S1P pathway genes were enriched with hypoxia-inducible factor 1-alpha, which indicated the close connection between the S1P pathway and the CIH process. In vitro, we confirmed that the endothelial cell apoptosis induced by CIH could be reversed by exogenous addition of S1P.</p> <p><bold>Conclusions</bold>: This study elucidated the mechanism of the S1P pathway in regulating cardiovascular injury caused by CIH and provided a new strategy for early intervention in people with cardiovascular dysfunction induced by hypoxia.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/rrlm-2024-00062024-01-29T00:00:00.000+00:00Challenges in prophylactic therapy with Emicizumab in patients with hemophilia A: Focus on monitoring testshttps://sciendo.com/article/10.2478/rrlm-2024-0003<abstract> <title style='display:none'>Abstract</title> <p>This study presents a transversal investigation that we performed at Fundeni hospital (Bucharest, Romania) into the therapeutic benefits and efficacy of Emicizumab, a non-factor therapy, in the context of hemophilia A. Ten patients diagnosed with hemophilia A were closely monitored using clinical and laboratory resources during Emicizumab treatment, with an average of 12.8 months. Among these patients, six exhibited anti-factor VIII inhibitors, changing the medical strategy and adding complexity to their clinical profiles. A comprehensive approach was adopted to assess the coagulation status of patients under Emicizumab therapy. The study employed several key coagulation monitoring tools, including thrombin generation time (TGT) and thrombelastography (TEG). These methodologies generated valuable results in evaluating the patients’ coagulation profiles during the treatment regimen. Additionally, traditional coagulation assays were utilized to gain a profound understanding of the overall coagulation dynamics and to evaluate the therapeutic response. During prophylaxis with Emicizumab all patients experienced a reduced number of bleeding events. Moreover, a subset of these patients underwent major surgical procedures (orthopedic joint replacements, cholecystectomy) with successful outcomes. These findings underscore the potential of Emicizumab therapy as an effective option for hemophilia A patients, including those with inhibitors. Our research provides physicians several insights, offering a potential avenue for improved patients’ care and treatment strategies that translate in enhanced quality of life for hemophilia A patients undergoing Emicizumab therapy.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/rrlm-2024-00032024-01-29T00:00:00.000+00:00Understanding the pathogenesis, clinical, laboratory diagnosis and treatment of the recent monkeypox virus outbreakhttps://sciendo.com/article/10.2478/rrlm-2024-0005<abstract> <title style='display:none'>Abstract</title> <p>Human mpox is a zoonotic disease, caused by the mpox virus (MPXV), that can spread either between animals and humans or humans and humans. In 1970 the first human case of mpox was reported in Zaire, Democratic Republic of the Congo (DRC). Other notable human mpox outbreaks in non-endemic countries were identified in June 2003 in the United States, in July 2021 in Dallas (USA), and the most recent one in May 2022 in Europe in the United Kingdom (UK). During the 2022 outbreak, sexual intercourse was observed to be the most prevalent transmission method, although other means should not be ignored, such as the involvement of respiratory droplets, exposure to surfaces and skin suspected of contamination. In the context of the current mpox outbreak, we consider it important and necessary to correctly identify the virus, use the proper laboratory testing for a correct diagnosis of mpox, understand the means of prophylaxis, and apply the correct treatment, highlighting these facts being the aim of this study.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/rrlm-2024-00052024-01-29T00:00:00.000+00:00Correlations of special AT-rich sequence binding protein 2 and chitinase-3-like protein-1 with sensitivity to paclitaxel chemotherapy for gastric cancerhttps://sciendo.com/article/10.2478/rrlm-2023-0030<abstract> <title style='display:none'>Abstract</title> <p><bold>Background</bold>: Our objective was to examine the associations between special AT-rich sequence binding protein 2 (SATB2) and chitinase-3-like protein-1 (CHI3L1) and the responsiveness to paclitaxel treatment in individuals with gastric cancer.</p> <p><bold>Methods</bold>: From March 2018 to October 2020, our hospital collected gastric cancer samples along with adjacent gastric mucosal tissues located more than 5 cm away from the cancerous margin. These samples were obtained from 90 patients who underwent chemotherapy regimens containing paclitaxel. To assess the rates of positive expression of CHI3L1 and SATB2 in gastric cancer and adjacent tissues, the immunohistochemical streptavidin-peroxidase (SP) technique was utilized.</p> <p><bold>Results</bold>: The positive expression rate of CHI3L1 was higher in gastric cancer tissues compared to adjacent tissues, while the positive expression rate of SATB2 was lower (P&lt;0.05). Risk factors that influenced the positive expression of CHI3L1 in gastric cancer tissues included the level of differentiation, tumor-node-metastasis (TNM) stage, and the presence of lymph node metastasis (OR&gt;1, P&lt;0.05). Additionally, the positive expression of SATB2 was also affected by TNM stage and lymph node metastasis, which were identified as risk factors (OR&gt;1, P&lt;0.05). In gastric cancer tissues, there was a negative correlation observed between the expressions of CHI3L1 and SATB2 (r&lt;0, P&lt;0.05). According to the analysis results of Kendall’s tau-b (K), it was found that the presence of CHI3L1 had an inverse relationship with the responsiveness to paclitaxel-based chemotherapy in gastric cancer (r=-0.498, P=0.000), while SATB2 exhibited a positive correlation with the sensitivity (r=0.513, P=0.000). During the 3-year follow-up after chemotherapy, the survival rate was 55.55% (50/90).</p> <p><bold>Conclusions</bold>: The findings indicate a strong correlation between SATB2 and CHI3L1 with the TNM stage, lymph node metastasis, response to paclitaxel-based chemotherapy, and the overall survival rate of individuals.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/rrlm-2023-00302023-10-28T00:00:00.000+00:00Analyzing serum tryptophan metabolites in patients with gestational diabetes mellitushttps://sciendo.com/article/10.2478/rrlm-2023-0027<abstract> <title style='display:none'>Abstract</title> <p><bold>Introduction</bold>: Although pregnancy is a physiological condition, the secretion of diabetogenic hormones such as growth hormone, corticotropin-releasing hormone, placental lactogen hormone, prolactin and progesterone from the placenta could lead to insulin resistance (IR). In Metabolic Syndrome, obesity and Type1&amp;2 diabetes, a shift in the kynurenine pathway (KP) towards IDO activation is observed. The activation of the IDO leads to the activation of the Aryl hydrocarbon receptor (AhR) and Interleukin-6 (IL-6) also, which may also induce some effects like insulin resistance, β-cell disfunction and increased gluconeogenesis. We hypothesized that the overactivation of IDO and some KP enzymes would be observed in GDM patients, in a similar manner to metabolic syndrome, prediabetes, and diabetes patients.</p> <p><bold>Methods</bold>: 50 patients and 50 controls, who applied to the Endocrinology outpatient clinic of Selcuk University Faculty of Medicine were included. Serum triptophan metabolite levels were measured with liquid chromatography tandem mass spectrometry.</p> <p><bold>Results</bold>: Tryptophan and KYNA values was found to be lower in the patient group diagnosed with GDM (p&lt;0.001 and p&lt;0.001, respectively). The levels of KYN, 3-OH AA, 3-OH-KYN and KTR were significantly higher in the patient group compared to the control group (p=0.008, p&lt;0.001, p=0.05 and p&lt;0.001, respectively).</p> <p><bold>Conclusions</bold>: Understanding the changes that occur in this pathway in GDM patients may provide insight into the development of the disease. Also these tests could be used as supplementary tests in gestational diabetes, which could assist in diagnosis and patient follow-up.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/rrlm-2023-00272023-10-28T00:00:00.000+00:00Associations of levels of high-molecular-weight adiponectin, secreted frizzled-related protein 5 and vascular endothelial growth factor-165 with diabetic retinopathyhttps://sciendo.com/article/10.2478/rrlm-2023-0028<abstract> <title style='display:none'>Abstract</title> <p><bold>Background</bold>: The pathogenesis of diabetic retinopathy (DR) remains unclear. The aim of the study was to explore the associations of DR with the levels of high-molecular-weight adiponectin (HMW-ADP), secreted frizzled-related protein 5 (SFRP-5) as well as vascular endothelial growth factor-165 (VEGF165).</p> <p><bold>Methods</bold>: Based on the diagnostic criteria for DR, non-DR (NDR), proliferative DR (PDR) plus non-proliferative DR (NPDR) groups were set up for type 2 diabetes mellitus (T2DM) patients (n=180) treated from January 2020 to March 2021. The control group consisted of another 60 healthy subjects undergoing physical examinations. Their clinical data were compared. Receiver operating characteristic curves were plotted to assess the predictive values of HMW-ADP, VEGF165 and SFRP-5 for DR. The predictive efficiency of the established nomogram model was assessed.</p> <p><bold>Results</bold>: The differences in age, fasting plasma glucose (FPG), T2DM duration, triglyceride (TG), hypertension history, high-density lipoprotein cholesterol (HDL-C), glycosylated haemoglobin (HbA1c), serum creatinine (Scr), homeostasis model assessment of insulin resistance (HOMA-IR), urine acid (UA), SFRP-5 HMW-ADP, and VEGF165 were significant between control and DR groups (P&lt;0.05). HMW-ADP, VEGF165 and SFRP-5 had predictive values for DR (AUC&gt;0.7), and the predictive efficiency of their combination was highest. The duration of T2DM, Scr, UA and VEGF165 were independent risk factors for DR, while HMW-ADP and SFRP-5 were protective factors (P&lt;0.05). Preferable discrimination and accuracy together with clinical applicability were obtained for the nomogram prediction model.</p> <p><bold>Conclusions</bold>: The three indicators as a whole have a high predictive value for DR, as potential indicators for the clinical screening of high-risk groups.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/rrlm-2023-00282023-10-28T00:00:00.000+00:00Proteomic patterns in glomerular research, a laser capture microdissection and liquid chromatography-tandem mass spectrometry approachhttps://sciendo.com/article/10.2478/rrlm-2023-0029<abstract> <title style='display:none'>Abstract</title> <p><bold>Introduction</bold>: Molecular techniques have the potential to shed light on glomerular diseases that conventional renal pathology may be unable to reveal. The aim of this study was to investigate whether proteomic patterns of glomeruli obtained from kidney biopsies can differentiate between minimal change disease (MCD), focal segmental glomerulosclerosis (FSGS) and control groups (CTR).</p> <p><bold>Methods</bold>: 18 formalin-fixed, paraffin-embedded (FFPE) renal biopsies comprising three groups of samples (CTR=3, MCD=6, FSGS=9) were subjected to label-free quantitative mass spectrometry. Glomeruli were excised from FFPE renal biopsies by laser capture microdissection (LCM) and, to increase both yield and protein identifications, single-pot solid-phase-enhanced sample preparation (SP3) digest method was applied. The samples were analyzed by mass spectrometry based shotgun proteomics.</p> <p><bold>Results</bold>: The proteome profiling resulted in the identification of a total of 723 proteins. Multivariate analysis provided several proteins important in the separation of the three groups. Pattern Hunter analysis revealed moderate and high correlation of proteins against CTR-FSGS-MCD or CTR-MCD-FSGS patterns. The most significant pathways involved were associated with nephrin family and cytoskeleton interactions, as well as laminin/extracellular matrix related proteins. Univariate analysis revealed 58 significant different proteins among the three groups. Signaling pathways of these proteins were also associated with nephrin family interactions and cytoskeleton organization.</p> <p><bold>Conclusions</bold>: This study demonstrates that mass spectrometry-based shotgun proteomic analysis of LCM glomeruli yields reproducible and quantitative data capable of discriminating between different disease conditions. Differentially expressed proteins provide insights into pathogenesis of glomerular disease.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/rrlm-2023-00292023-10-28T00:00:00.000+00:00Serum cytokine and chemokine profiles of patients with confirmed bacterial and viral meningitishttps://sciendo.com/article/10.2478/rrlm-2023-0023<abstract> <title style='display:none'>Abstract</title> <p><bold>Introduction</bold>: Cerebrospinal fluid (CSF) cytokines and chemokines have been reported by several studies as useful markers to discriminate bacterial and viral meningitis (BM and VM). This study aimed to investigate if serum cytokine and chemokine profiles could also differentiate BM from VM, thus circumventing the need for an invasive lumbar puncture.</p> <p><bold>Methods</bold>: Serum cytokines and chemokines were measured in 153 samples from patients with BM (n=58), VM (n=69), and controls (C, n=26) using multiplex assays. Cytokine and chemokine concentrations were compared among groups, correlation analyses were performed, and BM and VM cases classification based on cytokine and chemokine patterns was tested using a Machine Learning algorithm.</p> <p><bold>Results</bold>: IL-8, IL-1β, IL-6, IL-10, TNF-α, MCP-1, and ENA-78 showed a pronounced increase in the BM group compared to C (P&lt;0.01). Comparison of cytokines and chemokines in BM vs. VM showed significantly higher levels of MCP-1, IL-8, IL-1β, IL-6 and IL-10 (P&lt;0.01). Serum cytokine and chemokine concentrations were highly correlated in BM, being strongest for: MCP-1/IL-8, MCP-1/IL-1β, and IL-8/IL-1β (r=0.83; r=0.72; r=0.78, respectively). In VM, cytokine and chemokine correlations were weaker. The best predictors in the cytokine and chemokine pattern identified with a Random Forest algorithm for classifying BM vs VM were IL-8 and IL-10, and IL-6, but the specificity and sensitivity were low (85% and 69%, respectively).</p> <p><bold>Conclusion</bold>: Our results suggest significant changes in serum IL-6, IL-8, IL-10, and IL-1β in BM, but these mediators may have limited value in differentiating BM from VM.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/rrlm-2023-00232023-10-28T00:00:00.000+00:00The potential value of some adipokines and cytokines as diagnostic biomarkers for prostate cancerhttps://sciendo.com/article/10.2478/rrlm-2023-0031<abstract> <title style='display:none'>Abstract</title> <p><bold>Background</bold>: The role of Adipokines and proinflammatory cytokines is said to be crucial in the development of prostate cancer. Vaspin, Chemerin, Omentin, Interleukins IL-1β, interleukin-8 (IL8), Colony-stimulating factor (GM-CSF) and CC chemokine ligand 18 (CCL18) have all been proven to take part in tumor growth and progression.</p> <p>Aim of the study: The study aimed to explore circulating novel adipocytokines, such as serum of Vaspin, Chemerin and Omentin levels in patients with prostate cancer and to determine the level of selected proinflammatory cytokines (CCL18, IL-8, IL1, and GM-CSF).</p> <p><bold>Methods</bold>: Three groups were included in the current study: Group (1) :32 patients with metastatic prostate cancer who received chemotherapy, Group (2): 30 untreated patients with nonmetastatic prostate cancer, and Group (3): 30 healthy controls. ELISA technique was used to assess serum levels of Vaspin, Chemerin, Omentin, CCL18, IL-8, IL1, and GM-CSF).</p> <p><bold>Results</bold>: The Prostate cancer group exhibited higher serum levels of Vaspin, Chemerin, Omentin, CCL18, IL-8, IL1, and GM-CSF compared to the control group. Chemotherapy-treated patients exhibited significantly increased levels of the pro-inflammatory cytokines (IL-8) and Adipokines (Vaspin and Omentin) and decreased levels of the pro-inflammatory cytokines (IL-1) and Adipokines (Chemerin). The correlation analysis showed a significant positive correlation of serum Chemerin with Vaspin (r = 0.957, p-value&lt;0.0001), IL-8 (r = 0.9475, p-value &lt; 0.0001) and IL-1β (r = 0.7771, p-value &lt; 0.0029). Omentin and GS-CSF levels showed a non-significant positive correlation with Chemerin level (r = 0.1259; p = 0.6967).) and (r = 0.4247; p = 0.1688), respectively. While significant negative correlation was found between (Chemerin) with CCL-18 (r = –0.7916, p = 0.0022), serum Vaspin was significantly and negatively correlated with the levels of CCL-18 (r = –0.9349, p &lt; 0.0001), whereas there was a significant positive correlation between Vaspin level with IL-8 (R=0.9995, p &lt;0.0001); IL-1β(r = 0.561, p = 0.0057). The data demonstrated that Vaspin was positively and non-significantly correlated with the level of GS-CSF (r = 0.1437, p =0.656); serum Omentin was significantly and negatively correlated with the levels of GS-CSF (r = –0.8447, p = 0.0005), and CCL-18 (r= –0.7058, p = 0.0103), whereas there was a non-significant positive correlation between Omentin level with IL-8 (r = 0.4364, p = 0.1561). The data demonstrated that Omentin was negatively and non-significantly correlated with the level of IL-1β (r= –0.5366, p =0.0786).</p> <p><bold>Conclusions</bold>: This study indicated increased levels of serum Vaspin, Chemerin, Omentin, Interleukins IL-1β, interleukin-8 (IL8), Colony-stimulating factor (GM-CSF) and CC chemokine ligand 18 (CCL18) in patients with Prostate cancer. These findings suggest that the cytokines, and adipokines, whose levels were elevated in the chemotherapy-treated patients may be involved in the pathophysiology of prostate cancer. Vaspin, Chemerin and Omentin might play an important role in Prostate cancer progression through their association with Adipokines and proinflammatory cytokines. More studies are needed to investigate the possible role of Vaspin, Chemerin and Omentin as potential markers in the development of Prostate cancer.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/rrlm-2023-00312023-10-28T00:00:00.000+00:00Correlations of contrast-enhanced ultrasound parameters with free thyroxine, total thyroxine, thyroid peroxidase antibody and thyroglobulin antibody in patients with thyroid noduleshttps://sciendo.com/article/10.2478/rrlm-2023-0024<abstract> <title style='display:none'>Abstract</title> <p><bold>Background</bold>: We aimed to study the contrast-enhanced ultrasound features and parameters of patients with benign and malignant thyroid nodules.</p> <p><bold>Methods</bold>: A total of 154 patients diagnosed with thyroid nodules from January 2021 to December 2022 were selected as the subjects. They were divided into a benign nodule group (n=86) and a malignant nodule group (n=68). All patients were examined by contrast-enhanced ultrasound to analyze the features and the differences in parameters such as time to peak (Tp), mean transit time of contrast agent (MTT), peak intensity (PI), and area under curve (AUC).</p> <p><bold>Results</bold>: Using surgical histopathological results as the gold standard, the sensitivity, specificity, and accuracy of contrast-enhanced ultrasound for diagnosing thyroid nodules were 88.24% (60/68), 83.72% (72/86), and 85.71% (132/154), respectively. Compared to the benign nodule group, the levels of FT4, and TT4 significantly decreased, whereas those of TPOAb and TGAb significantly increased in the malignant nodule group (P&lt;0.05). MTT, PI, and AUC were positively correlated with FT4 and TT4 expressions but negatively correlated with TPOAb and TGAb expressions (P&lt;0.05). The diagnosis of thyroid nodules combined with contrast-enhanced ultrasound parameters had an AUC of 0.950, sensitivity of 93.42%, specificity of 87.90%, and 95% CI of 0.832-0.987, and the diagnostic efficiency exceeded those of diagnosis with single indicators (P&lt;0.05).</p> <p><bold>Conclusions</bold>: The contrast-enhanced ultrasound parameters MTT, PI, and AUC decreased in malignant thyroid nodules, being closely correlated with the thyroid function status. The images in combination with parameters of contrast-enhanced ultrasonography can be used to increase the accuracy of diagnosing benign and malignant thyroid nodules.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/rrlm-2023-00242023-10-28T00:00:00.000+00:00Updated insights into predictive biomarkers for response to cardiac resynchronization therapy - A literature reviewhttps://sciendo.com/article/10.2478/rrlm-2023-0025<abstract> <title style='display:none'>Abstract</title> <p>Cardiac resynchronization therapy (CRT) was shown to greatly improve outcomes in specific patients with heart failure, low ejection fraction and wide QRS complex; however post-therapeutic response is heterogeneous and currently difficult to predict. There is an increasing interest in identifying humoral biomarkers which could help stratify prognosis, and better identify responders. The aim of this review was to provide an overview of recent data regarding the predictive value of biomarkers for evaluating response to CRT. A definitive conclusion cannot currently be drawn due to disparate results, varying methodologies, relatively small-scale studies and lack of consensus in defining CRT response. More extensive comparable research is paramount to facilitate progress in this field.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/rrlm-2023-00252023-10-28T00:00:00.000+00:00Molecular and clinical aspects of HNSCC in the Republic of Moldovahttps://sciendo.com/article/10.2478/rrlm-2023-0026<abstract> <title style='display:none'>Abstract</title> <p><bold>Introduction</bold>: Complex molecular characterization and integrated approaches in the basic research of HNSCC provide new insights into the understanding and treatment of these tumors. Mutations in the TP53 gene, HPV infection, aberrant DNA methylation are just a few factors that have a direct link with the clinical and psychological condition of patients with this type of cancer. In the Republic of Moldova, these aspects are insufficiently studied.</p> <p><bold>Methods</bold>: The study included 128 patients with HNSCC from whom the following samples were collected: fresh tumor tissue, NAT, blood, and saliva. All samples, except saliva, were tested for 3 mutations in the TP53 gene, while DNA isolated from tumor tissue was also tested for global DNA methylation assessment. HPV genotypes were tested from saliva. HPV positive samples were retested from tumor tissue.</p> <p><bold>Results</bold>: Of the total analyzed samples for TP53 pathogenic variants, in 30 (23.44%) samples there were detected one or two mutations, and in 9 samples (7.03%) – it was detected the presence of two mutations simultaneously. HPV infection was detected in 17 samples (13.28%). Regarding global DNA methylation, in patients with a high degree of exposure to stress, a 44% lower level was observed (median 13.5 ng/ml) compared to those with moderate and low exposure (median 20.5 ng/ml ).</p> <p><bold>Conclusion</bold>: The most frequent mutation identified in the TP53 gene was the 524G&gt;A substitution and the frequency of high-risk HPV infection in HNSCC patients from Moldova was 13.28%. The high degree of stress exposure showed a lower level of global methylation.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/rrlm-2023-00262023-10-28T00:00:00.000+00:00Cybersecurity requirement of ISO 15189 - a simplified protocol for laboratorieshttps://sciendo.com/article/10.2478/rrlm-2023-0020ARTICLEtruehttps://sciendo.com/article/10.2478/rrlm-2023-00202023-07-28T00:00:00.000+00:00Clinical application value of Abbott Alinity analyzer in syphilis-specific antibody testinghttps://sciendo.com/article/10.2478/rrlm-2023-0017<abstract> <title style='display:none'>Abstract</title> <p><bold>Background</bold>: We aimed to investigate the clinical application value of Abbott Alinity analyzer in syphilis-specific antibody testing.</p> <p><bold>Methods</bold>: A total of 100 patients admitted from June 2021 to June 2022 for early syphilis diagnosis were selected and subjected to Treponema pallidum (TP) antibody testing by chemiluminescent microparticle immunoassay (CMIA) using Abbott Alinity analyzer. With TP particle agglutination (TPPA) retesting as the gold standard for syphilis diagnosis, the signal-to-cutoff (S/CO) ratio of the TP antibody testing was plotted into the receiver operating characteristic curve to determine the diagnostic value of CMIA and CLIA in detecting positive TP antibody and to identify the optimal cutoff point.</p> <p><bold>Results</bold>: In the case of S/CO ratio ≥7.00, the patients with positive CMIA were diagnosed with positive findings after TPPA confirmation. With the S/CO ratio of 1.00-4.99, the coincidence rate of CLIA with positive TPPA was 81.82% (45/55), and all patients with positive CLIA had positive results confirmed by TPPA test when the S/CO ratio was &gt;5.00. When the optimal cutoff value of S/CO ratio for TP was determined as 6.98 by CMIA, the sensitivity, specificity, and maximum area under the curve (AUC) were 94%, 88% and 0.91, respectively. At the optimal cutoff value (S/CO ratio: 4.56) determined by CLIA, the sensitivity was 84%, the specificity was 80%, and the maximum AUC was 0.84.</p> <p><bold>Conclusions</bold>: In the case of S/CO ratio ≥7.00, both methods have high sensitivity and specificity, which can directly give positive reports and shorten the sample turnaround time.</p> </abstract>ARTICLEtruehttps://sciendo.com/article/10.2478/rrlm-2023-00172023-07-28T00:00:00.000+00:00en-us-1