rss_2.0Revista Romana de Medicina de Laborator FeedSciendo RSS Feed for Revista Romana de Medicina de Laborator Romana de Medicina de Laborator 's Cover assessment, few paradigms and use cases<abstract> <title style='display:none'>Abstract</title> <p><bold>Background</bold>: The importance of applying the normality tests is underlined by the way of continuing the statistical protocol for numerical data within inferential statistics, respectively by the parametric or non-parametric tests that we will apply further on.</p> <p><bold>Methods</bold>: To check the calculation mode, we used sets of random values and we performed the normality assessment using statistical calculation programs. We took non-Gaussian data (n = 30, n = 50, n = 100, n = 500) and Gaussian data (n = 30, n = 50, n = 100, n = 500) for which we checked the normality of the data. Data chosen for this study were most representative for each batch (n).</p> <p><bold>Results</bold>: The application of normality tests to the data under study confirms that the data are non-Gaussian for the first data set. For the Gaussian data sample, the verification of normality is confirmed by the results.</p> <p><bold>Conclusion</bold>: For data up to 50 subjects, it is recommended to apply the Shapiro-Wilk test, but also to apply graphical methods to confirm the accuracy of the result. If the data samples have more than 50 values, the D’Agostino &amp; Pearson omnibus normality test should be applied and if the statistical program does not contain this test, the Shapiro-Wilk test can be applied (in the case of SPSS). Graphical methods, although they require some experience, are useful for identifying the normality of distributions with a small number of data.</p> </abstract>ARTICLE2022-07-18T00:00:00.000+00:00Associations of serum vitamin D and Fok I polymorphism of receptor gene with unexplained recurrent spontaneous abortion<abstract> <title style='display:none'>Abstract</title> <p><bold>Background</bold>: To investigate the associations of serum vitamin D and Fok I polymorphism of its receptor (VDR) with unexplained recurrent spontaneous abortion (URSA).</p> <p><bold>Methods</bold>: Ninety URSA patients and another 104 healthy pregnant women were selected as URSA and control groups, respectively. 25-Hydroxyvitamin D [25-(OH)D] level was detected by chemiluminescence. VDR gene Fok I polymorphism was analyzed by PCR, and the distribution of genotype frequency was calculated by Hardy-Weinberg equilibrium test. Association between Fok I polymorphism and susceptibility to URSA was investigated by logistic regression analysis.</p> <p><bold>Results</bold>: Gestational age, uterine height, waist circumference, 25-(OH)D level and proportions of Fok I FF and Ff genotypes were significantly lower in the URSA group (P&lt;0.05). Compared with ff genotype, risk of URSA reduced for Ff and FF genotypes. Compared with allele f, risk of URSA was lower for allele F. 25-(OH)D level of ff genotype was significantly lower in the URSA group, which was lower than that of FF genotype (P&lt;0.05). Compared with women with 25-(OH)D level &gt;30 ng/mL and F allele (FF+Ff), the risk of URSA increased 2.45-, 2.43- and 5.34-fold for those with 25-(OH)D level &gt;30 ng/mL and ff genotype, with 25-(OH)D level ≤30 ng/mL, and with ff genotype and 25-(OH)D level ≤30 ng/mL, respectively.</p> <p><bold>Conclusions</bold>: The 25-(OH)D level of the URSA group was significantly lower than that of normal pregnant women. Probably, VDR gene Fok I polymorphism is associated with URSA occurrence, and allele F decreases the risk. The risk of URSA dramatically increases in women with ff genotype and 25-(OH)D deficiency.</p> </abstract>ARTICLE2022-07-18T00:00:00.000+00:00Novel DCX pathogenic variant in a girl with subcortical band heterotopia<abstract> <title style='display:none'>Abstract</title> <p>Subcortical band heterotopia (SBH), is a brain malformation defined by symmetrical and bilateral heterotopic gray matter bands localized deep within the white matter, between the cortex and lateral ventricles. SBH is the result of abnormal neuronal migration, with improper positioning of the cortical neurons. DCX gene (doublecortin), a microtubule-associated protein with essential roles in neuronal migration and differentiation during brain development, is one of the main contributors to the X-linked Lissencephaly spectrum pathogenesis (OMIM #300067). DCX variants are responsible for SBH in females and isolated lissencephaly in males. Herein, we present a 7-year-old girl with a de novo frameshift variant in DCX gene, unreported by date. The patient has focal complex seizures with onset at 23 months of age, fully controlled with medication, mild tremor and coordination impairment of fine movements and some learning difficulties, otherwise with normal development. The brain magnetic resonance imaging revealed the presence of thick SBH. Direct sequencing of DCX gene revealed a pathogenic heterozygous cytosine duplication in exon 3; this frameshift variant leads to a premature stop codon in position 164 (p.Gln160Profs*5). The variant type and its predicted consequence at protein level correlates with the severity of radiological findings. The clinical presentation of our patient is, however, milder than expected. Our research expands the mutational spectrum of DCX gene in SBH females and provides a detailed clinical and imagistic description of the patient. This paper highlights the utility of single gene sequencing as a first-tier diagnostic test of patients with gene-specific phenotypic features.</p> </abstract>ARTICLE2022-07-18T00:00:00.000+00:00Downregulation of hsa-miR-4328 and target gene prediction in Acute Promyelocytic Leukemia<abstract> <title style='display:none'>Abstract</title> <p><bold>Introduction</bold>: Acute promyelocytic leukemia (APL) is defined by the PML-RARA fusion gene. APL treatment can have significant side effects, therefore the development of optimal therapeutic options is crucial. Although the study of miRNAs is still in its infancy, it has been shown that these molecules are involved in the pathogenesis of neoplasms by modulating the expression of target genes. miRNAs can be considered possible biomarkers in APL and can be used as therapeutic targets or as markers for the therapeutic response.</p> <p><bold>Objectives</bold>: The purpose of this study was to determine whether differentially expressed putative miRNAs that have RARA as a target gene could be considered reliable biomarkers for APL.</p> <p><bold>Methods</bold>: Using bioinformatics tools, a panel of 6 miRNAs with possible tropism for the RARA gene was selected from miRDB. We evaluated their expression levels in samples from patients with APL (n=20) or from healthy subjects without mutations in genes associated with leukemia or myeloproliferative diseases (n=21).</p> <p><bold>Results</bold>: All 6 putative miRNAs were identified using electrophoresis (hsamir-4299, hsa-mir-4328, hsa-mir-7851-3p, hsa-mir-6827-5p, hsa-mir-6867-5p, hsa-mir-939-5p). Of the six miRNAs, hsa-mir-4328 is deeply downregulated in subjects diagnosed with APL compared to healthy subjects, whereas hsa-mir-4299 and hsa-mir-7851-3p show small differences in expression between the two study groups, but without statistical significance. Our results suggest that hsa-mir-4328 may have a role in the pathogenesis of APL and may represent a new biomarker for this type of leukemia. Key Words: miRNA, APL, leukemia, bioinformatics.</p> </abstract>ARTICLE2022-07-18T00:00:00.000+00:00Expression of serum soluble Klotho protein in patients with renal damage induced by anti-neutrophil cytoplasmic antibody-associated small-vessel vasculitis and influence on prognosis<abstract> <title style='display:none'>Abstract</title> <p><bold>Background</bold>: Anti-neutrophil cytoplasmic antibody-associated small-vessel vasculitis (AASV) is an autoimmune disease with unclear pathogenesis, which causes damage to multiple organs and systems, renal failure or even death. We aimed to explore the expression of serum soluble Klotho protein in patients with AASV-induced renal damage and influence on prognosis.</p> <p><bold>Methods</bold>: A total of 330 AASV patients treated from June 2012 to June 2014 were divided into renal damage and non-renal damage groups. Clinical symptoms and laboratory examination results were compared. They were divided into Klotho &lt;935.05 pg/mL and ≥935.05 pg/mL groups, and renal damage and pathological indices were compared. Survival curves were plotted using Kaplan-Meier method, and 5-year and renal survival rates were compared.</p> <p><bold>Results</bold>: Compared with the non-renal damage group, the mean arterial pressure, urine protein and blood creatinine levels significantly rose, while the red blood cell count, hemoglobin, serum albumin, and Klotho protein levels declined in the renal damage group (P&lt;0.05). The optimal cut-off value of Klotho protein in assessing renal damage was 935.05 pg/mL. Compared with Klotho ≥935.05 pg/mL group, the levels of blood creatinine and urine protein significantly increased, and the proportion of normal glomeruli decreased, while that of fibrous crescents rose in Klotho &lt;935.05 pg/mL group (P&lt;0.05). The 5-year renal survival rate was significantly lower in Klotho &lt;935.05 pg/mL group than that in Klotho ≥935.05 pg/mL group (P&lt;0.05).</p> <p><bold>Conclusions</bold>: Klotho protein is lowly expressed in patients with renal damage induced by AASV as a potential marker for early diagnosis and prognostic evaluation.</p> </abstract>ARTICLE2022-07-18T00:00:00.000+00:00Expressions of TGF-β1 and VEGF in patients with acute myeloid leukemia and associations with prognosis<abstract> <title style='display:none'>Abstract</title> <p><bold>Background</bold>: To study the expressions of transforming growth factor-β1 (TGF-β1) and vascular endothelial growth factor (VEGF) in patients with acute myeloid leukemia (AML) and their values for prognosis.</p> <p><bold>Methods</bold>: A total of 120 AML patients treated from January 2015 to December 2018 were selected. Bone marrow mononuclear cells were isolated. The expressions of TGF-β1 and VEGF were detected by RT-PCR, and their associations with clinical characteristics were analyzed. The overall survival (OS) and disease-free survival (DFS) were assessed using the Kaplan-Meier method. The risk factors for prognosis were analyzed through the Cox proportional hazards model.</p> <p><bold>Results</bold>: The AML group had significantly lower relative expression of TGF-β1 and higher relative expression of VEGF than those of the control group (P&lt;0.05). TGF-β1 and VEGF levels were significantly correlated with white blood cell count, hemoglobin, platelets, and peripheral blood juvenile cells (P&lt;0.05). TGF-β1 level was higher and VEGF level was lower in the patients with complete response than those in the patients with partial response and no response (P&lt;0.05). Both OS and DFS of the patients with high TGF-β1 expression were better than those of the patients with low TGF-β1 expression, while they were also superior among the patients with low VEGF expression (P&lt;0.05). Platelets, TGF-β1 and VEGF were independent influencing factors for OS, and white blood cells, TGF-β1 and VEGF were independent influencing factors for DFS (P&lt;0.05).</p> <p><bold>Conclusions</bold>: AML patients have decreased expression of TGF-β1 and increased expression of VEGF, and such changes are closely associated with the prognosis of AML.</p> </abstract>ARTICLE2022-07-18T00:00:00.000+00:00Carbapenem resistance determinants in strains isolated from blood cultures-comparative analysis of molecular and phenotypic methods<abstract> <title style='display:none'>Abstract</title> <p><bold>Introduction:</bold> This study provides data on carbapenemases identified in carbapenem-resistant Klebsiella pneumoniae (CR-KP) isolated from blood-cultures by the multiplex molecular method.</p> <p><bold>Material and method</bold>: Between October 2016 and September 2017, 47 non-duplicate Klebsiella pneumoniae (KP) were isolated from blood cultures, from hospitalized patients in the Regional Institute of Gastroenterology and Hepathology, Cluj-Napoca, Romania. Identification and antimicrobial susceptibility tests (AST) were performed by Vitek 2 Compact. The combination disks test (CDT) was used for phenotypic analysis and the LightCycler® Multiplex DNA assay was used to detect and identify the carbapenemases by the LightCycler®z 480 Instrument. The following targets were chosen: bla<sub>KPC</sub>, bla<sub>NDM</sub>, bla<sub>GES</sub>, bla<sub>IMP</sub> and bla<sub>OXA-48</sub> genes and the Cobas® 4800 software variant 2.2.0 was used for the results interpretation.</p> <p><bold>Results</bold>: Taking into consideration the meropenem minimum inhibitory concentration (MIC), 29 KP were susceptible and 18 were not-susceptible (MIC≥0.5 µg ml-1). In the CR-KP group, the CDT identified OXA-48 (10/18) and KPC (7/18) producers. One isolate showed a noninterpretable profile. The multiplex molecular analyses confirmed the carbapenemases production as: 9 CR-KP were KPC and OXA-48 co-producers, 8 were OXA-48 and one was KPC producing strains. In CR-KP group, we found a significant correlation between the CDT and RT-PCR tests results, concerning KPC (p = 0.671). Eight phenotypic results were confirmed by molecular Light-Cycler® Multiplex DNA assay. For CR-KP co-producers (KPC and OXA-48), the CDT could indicate only one carbapenem-hydrolyzing enzyme.</p> <p><bold>Conclusion</bold>: This study highlights the CR-KP co-producers (OXA-48 and KPC). OXA-48-like is more frequently encountered in our area than other carbapenemases.</p> </abstract>ARTICLE2022-07-18T00:00:00.000+00:00 is a biomarker of cervical cancer prognosis and involved in immune infiltration<abstract> <title style='display:none'>Abstract</title> <p><bold>Background</bold>: Cervical cancer is the fourth commonly occurred cancer in women around the world. However, it still lacks effective approaches to improve current prognosis of cervical cancer and prevent metastasis.</p> <p><bold>Objective</bold>: We aim to discover a promising biomarker for cervical cancer prognosis by utilizing bioinformatics analysis.</p> <p><bold>Methods</bold>: Gene expression was analyzed by the datasets from The Cancer Genome Atlas Program-Cervical squamous cell carcinoma and endocervical adenocarcinoma (TCGA-CESC) dataset and three independent patient cohort datasets. Biological process and pathway enrichment were performed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analysis. Immune infiltration was analyzed through TISIDB tool.</p> <p><bold>Results</bold>: SPP1 gene was highly expressed in cervical cancer tissues. In addition, SPP1 was positively correlated to advanced CESC stages and nodal metastasis status. SPP1 co-expressed genes are mainly enriched in immunological processes. Furthermore, SPP1 expression is involved in immune infiltration level, in which several tumour infiltrating lymphocytes are correlated with SPP1. SPP1 overexpression promotes a wide spectrum of chemokines and immunoinhibiors which contribute to CESC progression.</p> <p><bold>Conclusions</bold>: SPP1 is a promising biomarker and a prognostic factor of CESC. Tumour infiltrating lymphocytes are also possibly regulated by SPP1. Our study suggests that investigation on SPP1 is a new direction for CESC therapy.</p> </abstract>ARTICLE2022-07-18T00:00:00.000+00:00Elbow Septic Arthritis caused by in a Newborn - Case Report and Literature Review<abstract> <title style='display:none'>Abstract</title> <p>Septic arthritis is a rare condition in children, especially in neonates. Early recognition and proper treatment are required to avoid serious complications. We present the case of a male newborn diagnosed with septic arthritis of the right elbow who was treated with repeated joint aspiration and i.v. antibiotics (Amikacin and Meropenem). Blood culture and joint cultures were positive for Klebsiella pneumoniae. The final outcome was good, with no apparent local or systemic complications. General considerations regarding this rare condition, etiology and treatment options are also included in the paper.</p> </abstract>ARTICLE2022-07-18T00:00:00.000+00:00Antibacterial Activity of Clove, Oregano, Thyme, Eucalyptus, and Tea Tree Essential Oils against and strains<abstract> <title style='display:none'>Abstract</title> <p><bold>Background</bold>: In view of the high recurrence rate of urinary tract infections and the increasing number of germs resistant to multiple antibiotics, the aim of the present study was to evaluate the antibacterial properties of clove, oregano, thyme, eucalyptus, tea tree essential oils (EOs) against 32 isolates of Escherichia coli and 28 isolates of Klebsiella pneumoniae from patients with urinary tract infections (UTI).</p> <p><bold>Methods</bold>: The agar disk diffusion method was used to assess the susceptibility of these isolates to essential oils and the minimal inhibitory concentration (MIC), and the minimal bactericidal concentration (MBC) were determined.</p> <p><bold>Results</bold>: Our results suggest that volatile phenols (such as carvacrol in oregano EO, thymol in thyme EO, and eugenol in clove EO) are more efficacious as antibacterial than non-aromatic compounds (such as eucalyptol in eucalyptus EO and terpinene derivatives in tea tree EO).</p> <p><bold>Conclusion</bold> : The oregano EO, followed by thyme appear to have the highest efficacy against Escherichia coli and Klebsiella pneumoniae isolates investigated.</p> </abstract>ARTICLE2022-07-18T00:00:00.000+00:00Effects of nitroglycerin combined with continuous regional arterial infusion on severe acute pancreatitis, triglyceride, inflammatory factors and prognosis<abstract> <title style='display:none'>Abstract</title> <p><bold>Background</bold>: To evaluate the effects of nitroglycerin combined with continuous regional arterial infusion (CRAI) on severe acute pancreatitis (SAP), triglyceride (TG), inflammatory factors and prognosis.</p> <p><bold>Methods</bold>: SAP patients were randomly divided into control and observation groups (n=169). The control group was treated with intravenous infusion of nitroglycerin once daily for 2 d, while the observation group was treated with nitroglycerin combined with CRAI for 7 d. Their hospitalization time, abdominal pain relief time, abdominal distension relief time and intestinal function recovery time were recorded. The levels of inflammatory factors, and TG were measured. Their liver and kidney functions, hemorheological indices, prognosis, and adverse reactions were evaluated.</p> <p><bold>Results</bold>: The observation group had significantly shorter hospitalization time, abdominal pain relief time, abdominal distension relief time, and intestinal function recovery time than those of the control group (P&lt;0.05). After treatment, the levels of interleukin-6, tumor necrosis factor-alpha, endothelin, thromboxane A2 and TG significantly decreased in contrast with those before treatment in both groups, especially in the observation group (P&lt;0.05). The hemorheological indices were significantly improved after treatment compared with those before treatment in both groups, and the observation group had better indices (P&lt;0.05). The Acute Physiology and Chronic Health Evaluation-II score declined significantly in both groups after treatment, and the observation group had a more obvious decrease (P&lt;0.05). During follow-up, similar incidence rates of adverse reactions were observed for both groups (P&gt;0.05).</p> <p><bold>Conclusion</bold>: Nitroglycerin combined with CRAI is prominently effective for treating SAP, which can facilitate the health recovery and reduce the incidence of microcirculation disturbance-induced adverse events such as organ failure.</p> </abstract>ARTICLE2022-05-09T00:00:00.000+00:00Associations of serum expressions of miR-499 and sex determining region Y-box 6 with prognosis of acute myocardial infarction patients<abstract> <title style='display:none'>Abstract</title> <p><bold>Background</bold>: To explore the associations of serum expressions of miR-499 and sex determining region Y-box 6 (SOX6) with major adverse cardiovascular and cerebrovascular events (MACCE) and prognosis of acute myocardial infarction (AMI) patients undergoing percutaneous coronary intervention (PCI).</p> <p><bold>Methods</bold>: The clinical data of 132 patients diagnosed from February 2016 to October 2019 were collected. Serum miR-499 and SOX6 expressions were detected by RT-qPCR. Optimal cut-off values were determined using receiver operating characteristic curves, based on which patients were divided into low and high miR-499 expression groups, and high and low SOX6 expression groups. Survival curves were plotted using Kaplan-Meier method, and the independent risk factors for MACCE were explored by multivariate logistic regression analysis. A nomogram model was established based on the factors and validated using internal data.</p> <p><bold>Results</bold>: AMI group had higher miR-499 expression and lower SOX6 expression than those of control group (P&lt;0.05). After PCI, miR-499 expression decreased and SOX6 expression increased (P&lt;0.05). Low miR-499 expression group had higher 3-year survival and MACCE-free rates than those of high miR-499 expression group (P&lt;0.05). Low SOX6 expression group had lower 3-year survival and MACCE-free rates than those of high SOX6 expression group (P&lt;0.05). AMI history, LVEF, CK-MB, miR-499 and SOX6 expressions were independent risk factors for MACCE (P&lt;0.05). The nomogram model had high accuracy for predicting overall survival, with a concordance index of 0.742 (95%CI=0.684-0.845).</p> <p><bold>Conclusions</bold>: AMI patients have increased serum expression of miR-499 and decreased expression of SOX6. High miR-499 expression is an independent risk factor for poor prognosis. The established nomogram model can be used to predict the risk of MACCE after PCI.</p> </abstract>ARTICLE2022-05-09T00:00:00.000+00:00MMP-2 and MMP-9 gene polymorphisms and risk of head and neck carcinomas<abstract> <title style='display:none'>Abstract</title> <p><bold>Background</bold>: Head and neck carcinomas (HNC) account for a majority of ear, nose and throat tumours. They account for 6.3% of all incident malignancies and 6.2 % of all deaths from cancer in Romania in 2020, the fifth most common cancer in this Eastern Europe country. Aim of the study: The aim of our study was to investigate the association between two MMP-2 and MMP-9 promoter gene polymorphisms and head and neck cancer.</p> <p><bold>Methods</bold>. We enrolled 142 subjects, 65 cancer patients, and 77 control subjects and tested them for MMP-2 -735 C/T and MMP-9 -1562 C/T polymorphisms by PCR-RFLP.</p> <p><bold>Results</bold>. Comparison between cancer patients and controls demonstrated the presence of MMP-2 -735 C/T and MMP-9 -1562 C/T in head and neck malignant tumours, with OR = 2.206 (95% CI 1.058-4.599, P = 0.03) for MMP-2 and OR = 2.748 (95% C.I. 1.262-5.981, P=0.009) for MMP-9 gene polymorphism. This means that the presence of T allele could be a risk factor for head and neck cancer development. The analysis included a stratification of studied groups by age and gender.</p> <p><bold>Conclusions</bold>. Both genotypes were associated with a significant risk for head and neck carcinomas in case of the presence of the T allele. MMP-2 -735 C/T (rs2285053) and MMP-9 -1562 C/T (rs3918242) gene polymorphism could be an important genetic marker for head and neck cancer susceptibility. This finding could be useful for genetic screening in head and neck carcinomas.</p> </abstract>ARTICLE2022-05-09T00:00:00.000+00:00Approaching Risk Management in Medical Laboratories<abstract> <title style='display:none'>Abstract</title> <p>Risk is one of the greatest challenges in a medical laboratory. Risk-based thinking is a concept that always preoccupies medical laboratory technicians. The objectives of this work were to bring forward the specialty standards recommendations for the implementation of risk management principles in medical laboratory, the accreditation requirement of ISO 15189:2012; to demonstrate that the understanding of the fundamental notions of the concept of risk, the effectively apply of the means of identifying, assessing and controlling risks, and the risk monitoring and handling through strategies of acceptance, elimination, transfer and mitigation of risks can ensure the continuous improvement processes. A model to approach to risk management in a medical laboratory establishes the inputs, the outputs, the techniques, and the activities carried out in each of the following sub-processes of the risk management process: risk management process planning, risk identification, risk analysis and evaluation, development of the risk response plan and risk monitoring, control and revision. The concepts of risk and risk management are applied accordingly to standards ISO 31000:2018 and ISO 22367:2020 of International Organization for Standardization (ISO). Risk management ensures that the fundamental requirements for healthy, proactive internal control of the medical laboratory are met.</p> </abstract>ARTICLE2022-05-09T00:00:00.000+00:00Clinical significance of serum HMGB1 in COPD and correlation with severity of airflow restriction and immune function<abstract> <title style='display:none'>Abstract</title> <p><bold>Background</bold>: To explore the serum HMGB1 levels in patients with smoking-induced chronic obstructive pulmonary disease (COPD) and the correlations with airflow restriction and immune function.</p> <p><bold>Methods</bold>: A total of 136 COPD patients were divided into mild, moderate and severe + extremely severe groups. Thirty-five healthy subjects were selected as control group. Serum HMGB1 levels were measured by ELISA, and the correlations with pulmonary and immune function indices were analyzed. Receiver operating characteristic (ROC) curve was plotted.</p> <p><bold>Results</bold>: PaO2, eosinophil count, FEV1/FVC, FEV1% pred, and IgA, IgM, IgG levels of COPD patients were lower than those of control group, and decreased with airflow restriction aggravation. PaCO2, leukocyte count, neutrophil percentage, modified British Medical Research Council (mMRC) scale and COPD Assessment Test (CAT) scores, D-Dimer (D-D), PCT, CRP and HMGB1 levels, myeloid dendritic cell (mDC) and plasmacytoid dendritic cell (pDC) counts, and mDCs/pDCs of COPD patients exceeded those of control group, and increased with airflow restriction aggravation (P&lt;0.05). HMGB1 levels of COPD patients were negatively correlated with FEV1/FVC, FEV1% pred, IgA, IgM and IgG levels and positively correlated with mDC count, pDC count and mDCs/pDCs (P&lt;0.0001). The area under ROC curve was 0.883, the optimal cutoff value was 3.63 ng/mL, and sensitivity and specificity were 86.7% and 85.9%, respectively.</p> <p><bold>Conclusions</bold>: Serum HMGB1 level in patients with smoking-induced COPD rises with airflow restriction aggravation and has significant correlations with the decline of pulmonary and immune functions, with high predictive value for COPD. HMGB1 is a potential biomarker for evaluating COPD progression.</p> </abstract>ARTICLE2022-05-09T00:00:00.000+00:00Crinum latifolium extract inhibits lipopolysaccharide-induced inflammation in human macrophages<abstract> <title style='display:none'>Abstract</title> <p><bold>Introduction</bold>: Crinum latifolium is a natural plant showing the anti-inflammatory effects. We aimed to evaluate the effects of Crinum latifolium extract on proinflammatory cytokine production and maturation of early and late endosomes in the lipopolysaccharide (LPS)-induced human macrophages at light microscopic and biochemical levels.</p> <p><bold>Material and Methods</bold>: The effects of Crinum latifolium extract in human macrophages stimulated with LPS was evaluated to optic densities of the lysosome-associated membrane protein-1 (LAMP-1) and early endo-some antigen 1 (EEA1) by immunohistochemistry staining and to level of the proinflammatory cytokine interleukin (IL)-6 by enzyme-linked immunosorbent assay (ELISA).</p> <p><bold>Results</bold>: Crinum latifolium group exhibited a significant decreased in the levels of IL-6 in the supernatant as compared to the LPS group for 2 and 72 hours (p&lt;0.0001). Crinum latifolium group exhibited a significant increased in the optic density of the EEA1 and LAMP-1 in coverslips as compared to the control and lipopolysaccharide groups after 2 and 72 hours (p&lt;0.0001).</p> <p><bold>Conclusions</bold>: Crinum latifolium may a therapeutic plant in the inflammatory diseases, such as sepsis, through anti-inflammatory effects, such as decrease in production of the proinflammatory cytokine and increase in maturations of the early and late endosomes in macrophages.</p> </abstract>ARTICLE2022-05-09T00:00:00.000+00:00Snapshot of resistance and virulence features in ESCAPE strains frequently isolated from surgical wound infections in a Romanian hospital<abstract> <title style='display:none'>Abstract</title> <p><bold>Background</bold>: The aim of this study was to investigate the phenotypic features (adherence, biofilm formation, virulence, antibiotic susceptibility) and the genetic background of antibiotic resistance in nosocomial ESCAPE strains consecutively isolated from surgical wound infections in hospitalized patients.</p> <p><bold>Methods</bold>: 86 bacterial strains consecutively isolated from various wound infections were analysed by their antibiotic resistance (antibiotic susceptibility testing and PCR for certain antibiotic resistance genes), virulence, biofilm formation and cellular adherence.</p> <p><bold>Results</bold>: The bacterial isolates were identified as: Enterobacterales (n = 39) including Escherichia coli (n = 9), Klebsiella pneumoniae (n = 14) Proteus mirabilis (n = 7), followed by Staphylococcus aureus (n = 26) and Enterococcus faecalis (n = 20). Compared to other isolates, S. aureus strains exhibited the highest capacity to produce soluble virulence factors and to develop biofilms in vitro, with significant differences between methicillin resistant and methicillin susceptible isolates. Among enterobacterial isolates, K. pneumoniae strains expressed the highest capacity to develop biofilms. The assessment of bacterial adherence to HeLa cells revealed that all bacterial strains adhered to the cellular substrata, showing various adherence patterns. E. faecalis strains exhibited a low soluble virulence factors profile, a lower capacity to adhere to epithelial cells and to develop biofilms.</p> <p><bold>Conclusions</bold>: The present study could contribute to the understanding of the pathology of infected wounds, depending on the etio-logical agents, providing data with positive impact on the therapeutic management of surgical wounds infections.</p> </abstract>ARTICLE2022-05-09T00:00:00.000+00:00Matrix-assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS) for subgingival bacteriome identification in a group of treated periodontitis patients: a case series<abstract> <title style='display:none'>Abstract</title> <p>Periodontitis is a chronic multifactorial polymicrobial infection, characterized by profound modifications of the composition and proportion of the subgingival microbiota. Microbiological laboratory tests are sometimes used in periodontal diagnosis and monitoring of treatment, but both conventional cultivation methods and molecular techniques have some major drawbacks. Therefore, other performant bacterial identification methods must be considered. The aim of the current study was to use Matrix-assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALTI-TOF MS) analysis in association with bacterial culture method to evaluate the modifications of the subgingival bacterial composition in periodontitis patients, before and after cause-related subgingival therapy. Subgingival plaque samples were collected from periodontal pockets before and after subgingival mechanical instrumentation and adjunctive local antimicrobial applications and were cultured in aerobic and anaerobic conditions. Microbial colonies were further assessed using MALDI-TOF-MS. A total of 36 bacterial strains were isolated from a group of 16 patients. All species from the orange complex were identified by MALDI-TOF MS. A marked reduction of detection frequency was observed in most bacterial strains, including the orange complex after cause-related periodontal treatment. The results of this study indicate that MALDI-TOF MS could be considered an accurate method for oral microbial identification and the cause-related periodontal treatment is useful for reducing the microbial burden.</p> </abstract>ARTICLE2022-05-09T00:00:00.000+00:00Proposal of a prediction model for prognosis of patients with acute myocardial infarction after percutaneous coronary intervention based on galectin-3 and soluble growth stimulating expressed gene 2 levels<abstract> <title style='display:none'>Abstract</title> <p>Background: To study the correlations of serum galectin-3 (Gal-3) and soluble growth stimulating expressed gene 2 (sST2) levels with prognosis of patients with acute myocardial infarction (AMI) after percutaneous coronary intervention (PCI).</p> <p>Methods: A total of 112 patients diagnosed from August 2015 to October 2017 were selected. They were followed up for 3 years. Based on major adverse cardiovascular events (MACEs) during follow-up, they were divided into MACE and non-MACE groups. Multivariate logistic regression analysis was performed to explore the independent risk factors for MACEs. A nomogram model was established using the factors and validated. The optimal cut-off values of Gal-3 and sST2 levels were determined by receiver operating characteristic curves. Kaplan-Meier method was used for survival analysis.</p> <p>Results: MACEs occurred in 78 patients during follow-up. Patients in the MACE group were more often hypertensive, had higher total cholesterol, uric acid, sST2 and Gal-3, and lower left ventricular ejection fraction (LVEF) (P&lt;0.05). CK-MB, sST2, Gal-3 and LVEF were the independent risk factors for MACEs (P&lt;0.05). The nomogram model established with these factors had high accuracy for predicting overall survival, and its concordance index (C-index) was 0.768 (95% confidence interval: 0.692-0.865). The prognosis of the patients with Gal-3 ≥12.57 μg/ mL and sST2 ≥18.56 ng/mL was poorer 3 years after PCI.</p> <p>Conclusions: The levels of serum Gal-3 and sST2 are the independent risk factors for MACEs in AMI patients following PCI, with high prognostic value.</p> </abstract>ARTICLE2022-05-09T00:00:00.000+00:00Non-inhibitory effects of the potent antioxidant from sp. on the glycation reaction<abstract> <title style='display:none'>Abstract</title> <p>When glucose and Amadori products are auto-oxidized, glycation occurs, resulting in the formation of early (Amadori) and late advanced glycation end products (AGEs), as well as free radicals. Glycation and an increase in free radical activity induce diabetic complications. Antioxidant and antiglycation compounds may aid in the prevention of oxidation and glycation. The goal of this study was to assess the antiglycation and antioxidant capacity of C-phycocyanin (C-PC) derived from Plectonema sp. The DPPH (1, 1-diphenyl-2-picrylhydrazyl), nitric oxide, hydroxyl radical scavenging activities and ferric ions reducing antioxidant power (FRAP) assays were used to assess antioxidant activity, while an in vitro bovine serum albumin-methyl glyoxal glycation (BSA-MG) model was used to assess glycation inhibitory potential. Glycation inhibition was measured using a variety of spectroscopic and biochemical parameters, including UV-visible &amp; fluorescence spectroscopy, ketoamine, carbonyl and hydroxymethyl furfural content, as well as free lysine &amp; free arginine estimations. In vitro, C-PC exhibited dose-dependent potent antioxidant activity, but lacked significant antiglycation potential. As a result, it is recommended that further studies be conducted to evaluate the antiglycation potential of C-PC.</p> </abstract>ARTICLE2022-05-09T00:00:00.000+00:00en-us-1