rss_2.0Medical Journal of Cell Biology FeedSciendo RSS Feed for Medical Journal of Cell Biologyhttps://sciendo.com/journal/ACBhttps://www.sciendo.comMedical Journal of Cell Biology 's Coverhttps://sciendo-parsed-data-feed.s3.eu-central-1.amazonaws.com/61dfb0b81792e62a88ea4466/cover-image.jpg?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20220927T211111Z&X-Amz-SignedHeaders=host&X-Amz-Expires=604800&X-Amz-Credential=AKIA6AP2G7AKP25APDM2%2F20220927%2Feu-central-1%2Fs3%2Faws4_request&X-Amz-Signature=c1fbfcdd41d79bc7db10e3cd56c89a544d65aeb6694bbc66e5a958fa3050f691200300ELABELA as a Marker of Gestational Complications – A Reviewhttps://sciendo.com/article/10.2478/acb-2022-0007<abstract> <title style='display:none'>Abstract</title> <p>Adipose tissue secretes dozens of biologically active molecules known as adipokines or adipocytokines. Apelin receptor early endogenous ligand (ELABELA, also known as ELA or APELA) is a circulating signaling protein expressed in placental tissue that binds to apelin receptors. The first animal experimental findings suggested that the ELABELA deficiency might be responsible for the pathogenesis of preeclampsia--like symptoms, i.e., hypertension and proteinuria in mice. Exogenous ELABELA supplementation reverted preeclampsia symptoms and normalized fetal birth weight in mice. Several in vitro studies confirmed that ELABELA supplementation could improve trophoblast cell functions such as invasiveness and proliferation capacity. Thus, the ELABELA axis could serve as the target of innovative therapies for gestational complications. Nonetheless, most human studies do not support the thesis that disturbances in ELABELA secretion in early pregnancy are associated with an increased risk of preeclampsia. Therefore, it is unlikely that ELABELA could serve as a novel early marker of preeclampsia in humans. Alterations in the ELABELA secretion have also been discovered among patients with other gestational complications such as GDM and fetal growth restriction.</p> </abstract>ARTICLE2022-07-09T00:00:00.000+00:00Heavy Eye Syndrome – Case Reporthttps://sciendo.com/article/10.2478/acb-2022-0011<abstract> <title style='display:none'>Abstract</title> <p>This paper aims to report clinical features, differential diagnosis and successful surgical outcome of a patient with myopic strabismus fixus, also known as Heavy Eye syndrome (HES). We present a case of a 47-year-old man who presented to the Ophthalmology Department with diplopia and poor vision. In the past, he had been diagnosed with Graves’ disease, high myopia, and secondary open-angle glaucoma. He had undergone orbital decompression and cataract surgery of both eyes. On examination, the patient had esotropia and hypotropia with limited abduction and elevation. The patient was ultimately diagnosed with HES. Yokoyama’s surgery combined with a medial rectus muscle recession in the right eye, were performed. The procedure reduced esotropia, hypotropia and improved ductions.</p> </abstract>ARTICLE2022-07-09T00:00:00.000+00:00Potential Role of LYN, CCL2, ITGB3 and IL6 Genes in the Immune Response of Porcine Buccal Mucosa Cellshttps://sciendo.com/article/10.2478/acb-2022-0008<abstract> <title style='display:none'>Abstract</title> <p>The genes considered in this study, namely, <italic>LYN, CCL2, ITGB3</italic> and <italic>IL6</italic> can be related to immune response in porcine buccal mucosa cells primary cultured <italic>in vitro</italic>. These genes are also responsible for, inter alia, cell migration, differentiation, proliferation and apoptosis, intracellular signal transduction, extracellular matrix binding and wound healing. A total of 20 pubertal crossbred Landrace gilts bred on commercial farms were used to obtain buccal mucosa cell cultures, which were harvested on the 7<sup>th</sup>, 15<sup>th</sup> and 30<sup>th</sup> day after initiation of the culture. Expression levels of <italic>LYN, CCL2, ITGB3</italic> and <italic>IL6</italic> were evaluated employing Real-Time Quantitative Polymerase Chain Reaction. All studied genes showed expression. The expression of <italic>CCL2</italic> on day 15 was the highest of all factors measured. The greatest difference between the measurements occurred in gene <italic>IL6,</italic> between 7<sup>th</sup> and 15<sup>th</sup> day, while the least difference between the measurements occurred in gene <italic>LYN</italic>, between 7<sup>th</sup> and 15<sup>th</sup> day. Moreover, on the 7<sup>th</sup> day, <italic>LYN</italic> presented the lowest expression among all studied genes. Although <italic>in vitro</italic> conditions are much more controlled than <italic>in vivo</italic> conditions, all the factors that may positively or negatively affect cultured cells still cannot be fully predicted. Nevertheless, <italic>LYN, CCL2, ITGB3</italic> and <italic>IL6</italic> are a valuable starting point for studying further immunological processes in oral mucosal epithelial cells. Given their high regenerative potential, research into them is a highly valuable source of information for future wound healing therapies, where immunological processes should be carefully considered.</p> </abstract>ARTICLE2022-07-09T00:00:00.000+00:00The Influence of L-Methionine, DL-Methionine, and a Methionine Hydroxy Analog on Proliferation and Differentiation Potential of Avian Myoblastshttps://sciendo.com/article/10.2478/acb-2022-0012<abstract> <title style='display:none'>Abstract</title> <p>Methionine is an essential amino critical to many cell functions including the synthesis of proteins. Supplementation of methionine in vivo is typically through L-methionine, DL-methionine, or a methionine hydroxy analog (MHA). The goal of this study was to compare the function of L-methionine, DL-methionine, and an MHA as a source of methionine to myoblasts in vitro. Avian myoblasts isolated from turkey embryos were plated in media containing varying concentrations of DL-methionine (DLM; 1.125 mg/mL or 0.56 mg/mL) or methionine hydroxy analog (MHA; 1.28 mg/mL or 0.64 mg/mL) as well as a methionine deficient negative control group and an L-methionine supplemented positive control group. The results of the proliferation assay exhibited cell division in the absence of methionine which was not significantly different than the positive control group. Results from the myoblast fusion assay revealed significantly greater myotube diameter between methionine supplemented groups compared to the methionine deficient negative control. The findings of this study show an ability for avian myoblasts to proliferate in the absence of methionine, the significance of which is discussed. Additionally, findings from the fusion assay suggest that DL-methionine and MHA are potential cost-effective substitutes for methionine supplementation during terminal differentiation of avian myoblasts.</p> </abstract>ARTICLE2022-07-09T00:00:00.000+00:00Ocular Manifestations of Pediatric Inflammatory Multisystem Syndromehttps://sciendo.com/article/10.2478/acb-2022-0010<abstract> <title style='display:none'>Abstract</title> <p>This article aims to present up-to-date information on ocular symptoms of pediatric multi-system inflammatory syndrome. The reviewers obtained the results based on a search of an electronic database. The pediatric multi-system inflammatory syndrome appears a few weeks after COVID-19 in children. The exact etiology remains unclear. It is diagnosed based on clinical and laboratory criteria. The most prevalent manifestation of the syndrome is non-purulent conjunctivitis (observed in around 50% of cases). The other ocular findings in the pediatric multi-system inflammatory syndrome can be; eyelid swelling, bilateral uveitis or vitreous hyperreflective dots in the posterior vitreous. The treatment of the ophthalmic symptoms is systemic and topical, targeting the enhanced inflammatory response of the organism and the presence of the given ocular findings.</p> </abstract>ARTICLE2022-07-09T00:00:00.000+00:00Difficulties in Treatment of Iridocorneal Endothelial Syndrome - Case Reporthttps://sciendo.com/article/10.2478/acb-2022-0009<abstract> <title style='display:none'>Abstract</title> <p>The iridocorneal endothelial syndrome manifests in three clinical types: Chandler syndrome, progressive iris atrophy, and Cogan-Reese syndrome. It is caused by the presence of abnormal corneal endothelium on the iris stroma and anterior chamber angle leading to usually unilateral, progressive iris atrophy, glaucoma, and/or corneal edema. The etiology is yet unclear. It affects mostly young adults, mostly females. Management of iridocorneal endothelial syndrome is complex: conservative and surgical, depending on the stage of the disease and intensity of present symptoms. A 30-year-old female with a medical history of the iridocorneal endothelial syndrome was reported to the Ophthalmology Department for consultation. Slit-lamp examination revealed iris atrophy and superior-nasal corectopia in the left eye. On gonioscopy, the angle was wide open in the right eye, but there were iridocorneal adhesions and incomplete angle-closure in the left eye. The patient was provided with maximum local therapy consisting of three anti-glaucoma medications. On later check-ups, the patient presented corneal edema and increased intraocular pressure. She was qualified to ExPress mini shunt trabeculectomy with mitomycin C. Two years later, a patient came to the clinic because of increased values of intraocular pressure (up to 59 mmHg), slit-lamp examination showed that the ExPress implant was congested with fragments of the corneal endothelial cells. Thanks to YAG iridotomy the implant was recanalized.</p> </abstract>ARTICLE2022-07-09T00:00:00.000+00:00Current application of exosomes in medicinehttps://sciendo.com/article/10.2478/acb-2022-0003<abstract> <title style='display:none'>Abstract</title> <p>Exosomes are a distinct type of extracellular vesicles that play a major role in intracellular transport and communication. Depending on the cell of origin, exosomes can contain diverse constituents of a cell, including DNA, RNA, lipids, metabolites, cytosolic and cell-surface proteins, playing important roles in a wide range of physiological and pathological processes. Due to these facts, they are subject of extensive research aiming at translating the knowledge into clinical approaches that are at the interface between nanomedicine and biopharmaceuticals.</p> <p>Their potential clinical use mostly revolves around the fields of diagnostics and drug delivery, especially important in treatment of cancer. The conventional and emerging methods of exosome isolation are either based on their physical properties (such as density and/or size) or their functions. However, the isolation approaches are still characterised by significant downsides, lacking standardisation, and ensuring purity. The review gives a critical overview on exosomes characteristics, isolation approaches and the potential that exosomes hold in developing new clinical approaches of modern medicine, highlighting the need for further research to fully grasp their potential and translate the knowledge into future therapeutic solutions.</p> </abstract>ARTICLE2022-04-10T00:00:00.000+00:00Approaches for in vitro culture of granulosa cells and ovarian follicleshttps://sciendo.com/article/10.2478/acb-2022-0006<abstract> <title style='display:none'>Abstract</title> <p>The in vitro culture of ovarian follicles or cumulus-oocyte complexes (COC) is used to study the factors that regulate follicular development and may have potential use in artificial reproductive technology (ART). Before ovulation, the follicle is formed by oocyte and cell populations known as granulosa cells (GCs). These cells build the internal and external mass of the follicular wall. Oocyte growth and proliferation of the surrounding cells depend on the gap junctions between the oocyte and the GCs. Maintenance of the optimal in vitro culture system allowing for preservation of follicle architecture and granulosa-oocyte interaction may be critical for success in vitro maturation of follicles. Recently many studies have focused on a culture of GCs, which have important functions related to steroidogenesis. Granulosa cells maintained in in vitro conditions exhibit stem cell properties making it important to consider in vitro culture (IVC) methods of the GC population.</p> </abstract>ARTICLE2022-04-10T00:00:00.000+00:00Follicular renewal and stemness potency of follicular cells depended of telomerase activity and TERT expression – short reviewhttps://sciendo.com/article/10.2478/acb-2022-0005<abstract> <title style='display:none'>Abstract</title> <p>Several hypotheses have been proposed, relating to the potential genesis of follicular cells in the ovarian niche. Reports using mice as an experimental model have suggested that the ovaries may contain stem cells that are likely involved in the formation of new follicles in adult reproductive life. Over recent years, various types of ovarian cells have been identified and described to confirm or disprove the existence of ovarian adult stem cells. Most research is focused on granulosa cells (GCs), which are essential for follicular development and maturation of female germ cells (oocytes). GCs exhibit the features of stem cells, such as expression of stem cell markers: OCT-4, Sox-2, Nanog as well as certain markers of mesenchymal stem cells, including CD29, CD44, CD90, CD105, CD117, and CD166. Another discovery in favor of the potential stemness of GCs is their ability to transdifferentiate towards other cell lines and high telomerase (TERT) activity in dividing compartments of the follicle during its maturation.</p> </abstract>ARTICLE2022-04-10T00:00:00.000+00:00Viability of bovine in vitro matured oocytes following ultra-rapid vitrificationhttps://sciendo.com/article/10.2478/acb-2022-0001<abstract> <title style='display:none'>Abstract</title> <p>The aim of the study was to examine viability of cattle oocytes after cryopreservation. Oocytes after <italic>in vitro</italic> maturation (IVM) were vitrified in minimum volume on the nickel electron microscopy grids by ultra-rapid cooling technique. After warming and subsequent in vitro fertilization the presumptive zygotes were cultured to reach the stage of the blastocyst (Bl). Several devitrified oocytes were processed for electron microscopy assay. Although, embryo cleavage and Bl percentages in the vitrified group were slightly lower than in the control group (P &lt; 0.05), the Bl total cell number (TCN), apoptosis and dead cell percentages did not differ between both groups. However, significant difference was found between day 7 (D7) and day 8 (D8) Bl in the TCN in control (108.0 vs. 90.5) and vitrified group (103.75 vs 98.14). Electron microscopy of frozen oocytes revealed slight reversible injuries in mitochondria and the smooth endoplasmic reticulum (SER), nevertheless, the development of devitrified oocytes to the Bl stage was comparable to those in fresh oocytes. In conclusion, higher proportion of slower developing Bl (D8) compared to D7 Bl may be related to the mentioned minor damages of some organelles in vitrified oocytes.</p> </abstract>ARTICLE2022-04-10T00:00:00.000+00:00Silica nanoparticles in targeted human cancer therapyhttps://sciendo.com/article/10.2478/acb-2022-0002<abstract> <title style='display:none'>Abstract</title> <p>One of the leading causes of death across the world is cancer. Despite massive attempts to develop efficient chemotherapy medications, there is still a significant toxicity and selectivity problem. We are looking for novel therapies and preventative strategies due to the toxicity of contemporary chemotherapy and cancer cell resistance to anticancer drugs. The structure and molecular characteristics of Na2SiO3 nanoparticles were investigated using density-functional theory calculations at the B3LYP/6-311G** level. The study looked at engineering qualities and several molecular recipes like HOMO, LUMO, and Egap in order to figure out how to arrange molecules as a powerful antioxidant, and hence the majority of the compounds are anticancer. We discovered that Na2SiO3 gel particles are responsible for antioxidant activity, implying that it can be employed as an antioxidant and anticancer for cancer prevention and treatment.</p> </abstract>ARTICLE2022-04-10T00:00:00.000+00:00Changes of the zona pellucida patterns during oocyte maturation, fertilization and embryo development in mammals: mini-reviewhttps://sciendo.com/article/10.2478/acb-2022-0004<abstract> <title style='display:none'>Abstract</title> <p>The mammalian zona pellucida (ZP) is an extracellular matrix that surrounds immature and mature oocytes and early embryos until the stage of a blastocyst and its implantation. This mini-review summarizes basic information on the ZP and its morphologic and functional changes during <italic>in vitro</italic> oocyte maturation and fertilization and <italic>in vivo</italic> pre-implantation embryo development.</p> </abstract>ARTICLE2022-04-10T00:00:00.000+00:00Aortocoronary conduits may show a different inflammatory response - comparative study at transcript levelhttps://sciendo.com/article/10.2478/acb-2020-0003<abstract><title style='display:none'>Abstract</title><p>Coronary artery bypass grafting (CABG), together with percutaneous coronary intervention (PCI), are both still the most efficient procedures for myocardial revascularization to treat advanced coronary artery disease (CAD). Donor blood vessels used in CABG are usually the internal thoracic artery (ITA) and saphenous vein (SV). The importance of inflammation and inflammatory pathways in graft patency is well established. Nevertheless, not all molecular mechanisms underlying the inflammatory process appear to be clear. Employing the expressive microarray approach to analyze the transcriptome of both venous and arterial grafts, five GO BP terms has been selected: “cellular response to interferon-gamma”, “inflammatory response”, “interferon-gamma-mediated signaling pathway”, “response to interferon-gamma” and “positive regulation of inflammatory response”. This study aimed to evaluate potential molecular factors that could be characteristic markers for both SV and ITA conduits.</p><p><bold>Running title</bold>: Aortocoronary conduits may show a different inflammatory response</p></abstract>ARTICLE2020-04-29T00:00:00.000+00:00Cell-based approaches in drug development – a concise reviewhttps://sciendo.com/article/10.2478/acb-2020-0005<abstract><title style='display:none'>Abstract</title><p><italic>In vitro</italic> models represent an alternative technique to <italic>in vivo</italic> or <italic>ex vivo</italic> studies in the drug development process. Cell-based assays are used to measure the level of proliferation and toxicity, as well as activation of signalling pathways and changes in morphology in cultivated cells. The studies conducted <italic>in vitro</italic> are aimed to estimate the newly synthesised drugs’ ability to permeate biological barriers and exert their therapeutic or cytotoxic effects. However, more than half of all studied drugs fail in the second or third phase of clinical trials due to a lack of confirmed efficacy. About a third of drugs fail because of safety issues, such as unacceptable levels of toxicity. To reduce attrition level in drug development, it is crucial to consider the implementation of translational phenotypic assays as well as to decipher various molecular mechanisms of action for new molecular entities. In this review, we summarise the existing cell-based methods most frequently used in the studies on drugs, taking into account their advantages and drawbacks.</p><p><bold>Running title</bold>: Cell-based approaches in drug development</p></abstract>ARTICLE2020-04-29T00:00:00.000+00:00New molecular markers involved in immune system homeostasis and hemopoietic organ development are differentially regulated during oocytes in vitro maturationhttps://sciendo.com/article/10.2478/acb-2020-0004<abstract><title style='display:none'>Abstract</title><p>The growth and maturation of the oocyte is a dynamic process which requires a variable supply of hormones, growth factors and energy. These needs are met partially by the surrounding somatic cells and the cumulus-oocyte complex, which communicate bi-directionally via gap junctions. Identifying and analyzing protein expression in the oocyte can provide insight in its development and growth. Further, like bone marrow stem cells, if relevant marker genes are found in oocytes, there is a potential for the oocyte to be manipulated into becoming hemopoietic stem cells. In this study, porcine oocytes were isolated and subjected to microarray analysis to compare the oocyte gene expression in vivo and in vitro maturation (IVM). Genes identified belonged to both ‘hemopoietic or lymphoid organ development’(GO:0048534) and ‘immune system development’ (GO:0002520), and the markers can be used to identify several activities such as cell migration, neurogenesis and proliferation. The following are the identified genes and all were downregulated after IVM to varying degrees: ID2, VEGFA, TGFBR3, INHBA, CDK6, BCL11A, MYO1E, ITGB1, EGR1, NOTCH2, SPTA1, KIT and TPD52. Our results should provide new markers to further investigate oocyte development and growth regulation.</p><p><bold>Running title</bold>: Markers of hemopoietic organ development</p></abstract>ARTICLE2020-04-29T00:00:00.000+00:00New markers of human cumulus oophorus cells cultured in vitro – transcriptomic profilehttps://sciendo.com/article/10.2478/acb-2020-0007<abstract><title style='display:none'>Abstract</title><p>The presence of CCs around the oocyte after ovulation is one of the key elements contributing to oocyte developmental competence. In the presented study, we used CCs from 12 patients aged 18-40 diagnosed with infertility. After harvesting cells on day 1, 7, 15 and 30 of culture, total RNA was isolated and transcriptomic analysis was performed. The DAVID software indicated the following GO BP terms: “cell junction organization”, “cell migration”, “cell morphogenesis involved in differentiation”, “cell morphogenesis” and “cell motility”. Of the genes belonging to all ontological groups, the most downregulated were: SLC7A8, DFNB31, COL1A1, CDC42SE1, TGFBR3, HMGB1, with the most upregulated genes being: ANXA3, KIAA1199, HTR2B, VCAM1, DKK1.</p><p>While many studies focus on attempts to obtain fully competent oocytes, scientists still have difficulty attaining adequate results in vitro. Lack of adequate knowledge often results in low in vitro fertilization efficiency. Therefore, our research focuses on CCs cells, thanks to which the oocyte most likely acquires developmental competence. The main purpose of the study was to identify the potential molecular markers responsible for cell junction organization, migration, differentiation, morphogenesis and motility.</p><p><bold>Running title</bold>: New markers of human cumulus oophorus cells cultured <italic>in vitro</italic></p></abstract>ARTICLE2020-04-29T00:00:00.000+00:00In search of markers useful for evaluation of graft patency - molecular analysis of ‘muscle system process’ for internal thoracic artery and saphenous vein conduitshttps://sciendo.com/article/10.2478/acb-2020-0002<abstract><title style='display:none'>Abstract</title><p>Coronary artery bypass graft (CABG) is the surgical method most commonly used to treat coronary artery disease (CAD). The vessels that are used in CABG are usually the internal thoracic artery (ITA) and the saphenous vein (SV). Transplant patency is one of the most important factors affecting transplant success. In this study, we used an expressive microarray method, approved by RT-qPCR, for transcriptome analysis of arterial and venous grafts. In the search for potential molecular factors, we analyzed gene ontologies of different expression based on the muscular system. Among interesting groups, we distinguished muscle cell proliferation, muscle contraction, muscle system process, regulation of smooth muscle cell proliferation and smooth muscle cell proliferation. The highest increase in gene expression was observed in: ACTN2, RBPMS2, NR4A3, KCNA5, while the smallest decrease in expression was shown by the P2RX1, KCNH2, DES and MYOT genes. Particularly noteworthy are the ACTN2 and NR4A3 genes, which can have a significant impact on vascular patency. ACTN2 is a gene that can affect the formation of atherosclerotic plaques, while NR4A3 occurs in 4 of the 5 ontological groups discussed and can affect the inflammatory process in the blood vessel. To summarize, the presented study provided valuable insight into the molecular aspects characterizing the vessels used in CABG, and in particular identified genes that may be the target for further studies on duct patency.</p><p><bold>Running title</bold>: CABG grafts’ molecular analysis of ‘muscle system process’</p></abstract>ARTICLE2020-04-29T00:00:00.000+00:00The processes of homeostasis, chemotaxis and organic and inorganic response are significantly up-regulated during short-term oral mucosal cells in vitro cultivationhttps://sciendo.com/article/10.2478/acb-2020-0006<abstract><title style='display:none'>Abstract</title><p>Mucous membranes appear in various parts of the whole body performing similar functions. However, they differ based on where the mucosa is located. It functions as a barrier in such systems as: respiratory, urogenital and digestive . In this study we will be focusing strictly on the oral mucosa. Keratinocytes and fibroblasts, which mainly form the structure of the oral mucosa, are subjected to numerous factors. Being one of the million parts that build the animal organism, they are involved in various processes. In this study, we will try to confirm that in the in vitro culture of oral mucosa cells, the expression of our selected genes undergoes significant changes which are tied to such processes as: homeostasis, chemotaxis and organic/inorganic response of the organism. For this study, 20 pubertal crossbred Landrace gilts were used. After slaughter, samples of buccal pouch mucosa were obtained and transported to the laboratory. The excised tissue was prepared and processed due to protocols. The final pellet was resuspended in supplemented DMEM. Once the cultures attained 70–80% confluency, they were passaged. Total RNA from each pooled sample was subjected to two rounds of sense cDNA amplification. The cDNA was processed on microarrays. Analysis of the scanned arrays was performed. The files were imported into downstream data analysis software. The DAVID analysis showed that differently expressed genes belongs to 56 Gene ontology groups. In this paper we focused on “cellular divalent inorganic cation homeostasis”, “chemical homeostasis”, “chemotaxis”, “homeostatic process” and “response to organic substance” GO BP terms. These sets of genes were subjected to hierarchical clusterization procedure. In summary, the data we collected showed primarily changes in gene expression that occurred in the thirty-day cell culture of oral mucosa tissue. We assume that indicated genes could be new gene markers for studied processes.</p><p><bold>Running title</bold>: Homeostasis in oral mucosa cells</p></abstract>ARTICLE2020-04-29T00:00:00.000+00:00The genes regulating maintenance of cellular protein location are differentially expressed in porcine epithelial oviductal cells during longterm in vitro cultivationhttps://sciendo.com/article/10.2478/acb-2019-0010<abstract><title style='display:none'>Abstract</title><p>The oviduct is a part of female reproductive tract that is essential for successful fertilization and early embryo development. It is lined with epithelium consisting of two types of cells: ciliated and secretory. The primary function of ciliated oviductal epithelial cells (OECs) is to support the transport of gametes and embryos through the ovary, whereas secretory OECs produce components of the oviductal fluid. Undoubtedly, the oviductal epithelium plays a major part in the early aspects of pregnancy development, from providing an optimal environment for gametes and embryos to supporting fertilization. Therefore, our aim was to gain a better insight into the genetic changes underlying function of these cells. We have harvested OECs from crossbred gilts (n=45), at the age of about nine months and which displayed two regular estrous cycles, and established long-term primary culture of porcine OECs. Microarray analysis was utilized to determine differentially expressed genes during day 1, 7, 15 and 30 of cultivation, with our results revealing54 differentially expressed genes belonging to three ontology groups: „maintenance of location”, „maintenance of protein location” and „maintenance of protein location in cell”. Since the biochemistry and morphology of epithelial cells may change during long term cultivation, we conclude that our results are a reflection of these changes and help to shed a light on porcine OECs properties in <italic>in vitro</italic> environment.</p><p><bold>Running title:</bold> Maintenance of cellular protein location in porcine epithelial oviductal cells</p></abstract>ARTICLE2019-10-12T00:00:00.000+00:00Genes encoding proteins regulating fatty acid metabolism and cellular response to lipids are differentially expressed in porcine luminal epithelium during long-term culturehttps://sciendo.com/article/10.2478/acb-2019-0008<abstract><title style='display:none'>Abstract</title><p>Among many factors, the epithelium lining the oviductal lumenis very important for the development of the oocyte and its subsequent fertilization. The oviductal epithelium is characterized by the presence of ciliary cells, supporting the movement of cumulus-oocyte complexes towards the uterus. By interacting with the semen, the epithelium of the fallopian tube makes the sperm acquire the ability to fertilize. So far, the exact molecular mechanisms of these changes have not been known. Hence, understanding the metabolism of oviduct epithelial cells and the level of expression of individual groups of genes seems to be a way to deepen the knowledge about the broadly understood reproduction.</p><p>In our research, we decided to culture oviductal epithelial cells (OECs) <italic>in vitro</italic> for a long period of time. After 24h, 7, 15 and 30 days, the OECs were harvested, with their RNA isolated. Transcriptomic changes were analyzed using microarrays. The “cellular response to lipid” group was represented by the following genes: <italic>MUC1, CYP24A1, KLF4, IL24, SNAI2, CXCL10, PPARD, TNC, ABCA10</italic>, while the genes belonging to the “cellular lipid metabolic processes” were: <italic>LIPG, ARSK, ACADL, FADS3, P2RX7, ACSS2, PPARD, KITLG, SPTLC3, ERBB3, KLF4, CRABP2</italic>. Additionally, PPARD and ACADL were members of the “fatty acid beta-oxidation” ontology group. Our study describes genes that are not directly related to fertility processes. However, significant changes in their expression in <italic>in vitro</italic> cultured OECs may indicate their usefulness as markers of OECs’ physiological processes.</p><p><bold>Running title:</bold> Fatty acids changes in porcine oviductal epithelial cells in in vitro cultivation</p></abstract>ARTICLE2019-10-12T00:00:00.000+00:00en-us-1