rss_2.0Journal of Electrical Bioimpedance FeedSciendo RSS Feed for Journal of Electrical Bioimpedance of Electrical Bioimpedance 's Cover the skin biophysical parameters among coronavirus patients for three days in a row: a preliminary study<abstract> <title style='display:none'>Abstract</title> <p>The coronavirus epidemic 2019 is spreading all over the world now. Several parameters are used to monitor the status of hospitalized patients; however, monitoring variations in biophysical properties of the skin has not been investigated yet. In this preliminary study, we seek to monitor skin biophysical parameters among coronavirus patients for three days in a row. Skin moisture, pH, sebum, and temperature during the three days were monitored in 30 coronavirus patients by using non-invasive portable instruments. Skin biophysical parameters were increased on the third day of monitoring compared to the first one. In addition, the increase in both skin moisture and temperature were statistically significant. According to the results of this preliminary study, skin biophysical parameters changed (increased) during the specified period in which the patients were monitored. However, changes in skin sebum content and pH were not significant. These skin parameters need to be further investigated until we know their indication ability for the health condition of coronavirus patients in clinical applications.</p></abstract>ARTICLE2022-05-20T00:00:00.000+00:00Influence of acute water ingestion and prolonged standing on raw bioimpedance and subsequent body fluid and composition estimates<abstract> <title style='display:none'>Abstract</title> <p>This study evaluated the influence of acute water ingestion and maintaining an upright posture on raw bioimpedance and subsequent estimates of body fluids and composition. Twenty healthy adults participated in a randomized crossover study. In both conditions, an overnight food and fluid fast was followed by an initial multi-frequency bioimpedance assessment (InBody 770). Participants then ingested 11 mL/kg of water (water condition) or did not (control condition) during a 5-minute period. Thereafter, bioimpedance assessments were performed every 10 minutes for one hour with participants remaining upright throughout. Linear mixed effects models were used to examine the influence of condition and time on raw bioimpedance, body fluids, and body composition. Water consumption increased impedance of the arms but not trunk or legs. However, drift in leg impedance was observed, with decreasing values over time in both conditions. No effects of condition on body fluids were detected, but total body water and intracellular water decreased by ~0.5 kg over time in both conditions. Correspondingly, lean body mass did not differ between conditions but decreased over the measurement duration. The increase in body mass in the water condition was detected exclusively as fat mass, with final fat mass values ~1.3 kg higher than baseline and also higher than the control condition. Acute water ingestion and prolonged standing exert practically meaningful effects on relevant bioimpedance variables quantified by a modern, vertical multi-frequency analyzer. These findings have implications for pre-assessment standardization, methodological reporting, and interpretation of assessments.</p></abstract>ARTICLE2022-05-20T00:00:00.000+00:00Identification of contractions from Electrohysterography for prediction of prolonged labor<abstract><title style='display:none'>Abstract</title><p>The analysis of the uterine electrical activity and its propagation patterns could potentially predict the risk of prolonged/arrested progress of labor. In our study, the Electrohysterography (EHG) signals of 83 participants in labor at around 3-4 cm of cervical dilatation, were recorded for about 30 minutes each. These signals were analyzed for predicting prolonged labor. Out of the 83 participants, 70 participants had normal progress of labor and delivered vaginally. The remaining 13 participants had prolonged/ arrested progress of labor and had to deliver through a cesarean section. In this paper, we propose an algorithm to identify contractions from the acquired EHG signals based on the energy of the signals. The role of contraction consistency and fundal dominance was evaluated for impact on progress of the labor. As per our study, the correlation of contractions was higher in case of normal progress of labor. We also observed that the upper uterine segment was dominant in cases with prolonged/arrested progress of labor.</p></abstract>ARTICLE2022-03-31T00:00:00.000+00:00Opinion: The future of electrical impedance tomography measurement of living with impedance spectroscopy<abstract><title style='display:none'>Abstract</title><p>Impedance spectroscopy is a useful tool for non-invasive and real time measurements of cell suspensions and a variety of biological tissues. The objective of this study was the investigation of the dielectric properties of living aquatic worms (<italic>Lumbriculus variegatus</italic>) using impedance spectroscopy in a frequency range between 100 Hz and 10 MHz. We demonstrate a linear relation between the worm biomass and the phase response of the signal thereby providing a quick and precise method to determine the biomass of aquatic worms <italic>in situ</italic>. Possible applications for non-destructive online biomass monitoring of aquatic worms and other aqueous organisms are discussed. Furthermore, we show that groups of worms fed different diets can be distinguished by the method presented. These results reveal a close relationship between the nutritional composition of the worms and the measured phase response. We also demonstrate that the phase response at 90 kHz does not depend on the worm size. In contrast, the response function for the signal at 440 Hz reveals a linear correlation of average individual worm size and phase. Therefore, we conclude that the measured phase response at 90 kHz qualifies as a measure of the total amount of worm biomass present in the measuring cell, whereas the phase measurement at 440 Hz can be used to estimate the average individual worm size.</p></abstract>ARTICLE2014-12-03T00:00:00.000+00:00Statistical methods for bioimpedance analysis<abstract><title style='display:none'>Abstract</title><p>This paper gives a basic overview of relevant statistical methods for the analysis of bioimpedance measurements, with an aim to answer questions such as: How do I begin with planning an experiment? How many measurements do I need to take? How do I deal with large amounts of frequency sweep data? Which statistical test should I use, and how do I validate my results? Beginning with the hypothesis and the research design, the methodological framework for making inferences based on measurements and statistical analysis is explained. This is followed by a brief discussion on correlated measurements and data reduction before an overview is given of statistical methods for comparison of groups, factor analysis, association, regression and prediction, explained in the context of bioimpedance research. The last chapter is dedicated to the validation of a new method by different measures of performance. A flowchart is presented for selection of statistical method, and a table is given for an overview of the most important terms of performance when evaluating new measurement technology.</p></abstract>ARTICLE2014-04-16T00:00:00.000+00:00The feasibility of using compression bioimpedance measurements to quantify peripheral edema<abstract><title style='display:none'>Abstract</title><p>The accurate assessment of body fluid volume is important in many clinical situations, especially in the determination of “dry weight” in a dialysis setting. Currently, no clinically applicable diagnostic system exists to determine the mechanical properties that accurately characterize peripheral edema in an objective and quantitative manner. We have developed a method for quantifying the impact of compression on the electrical properties of tissue by measuring stress-induced changes in bioimpedance (BIS). Using this method, we simultaneously measured the impedance and mechanical response of a tissue mimicking material (tofu) under both quasi-static and dynamic loading conditions. Our results demonstrate a temporal quantification of viscoelastic properties using a viscoelastic phantom tissue model.</p></abstract>ARTICLE2014-12-25T00:00:00.000+00:00Impedance Ratio Method for Urine Conductivity-Invariant Estimation of Bladder Volume<abstract><title style='display:none'>Abstract</title><p>Non-invasive estimation of bladder volume could help patients with impaired bladder volume sensation to determine the right moment for catheterisation. Continuous, non-invasive impedance measurement is a promising technology in this scenario, although influences of body posture and unknown urine conductivity limit wide clinical use today. We studied impedance changes related to bladder volume by simulation, in-vitro and in-vivo measurements with pigs. In this work, we present a method to reduce the influence of urine conductivity to cystovolumetry and bring bioimpedance cystovolumetry closer to a clinical application.</p></abstract>ARTICLE2014-09-09T00:00:00.000+00:00Simplified estimation of membrane potentials induced by high-frequency electric signals<abstract><title style='display:none'>Abstract</title><p>In this paper we show why the poorly conducting cytoplasmic membranes have little effect on the overall impedance of the tissue above a certain frequency, and derive an estimate of this upper frequency. It is further shown that the induced transmembrane potentials at different sites over the membrane can be found through a simple formula for frequencies above the threshold, without the need to analytically or theoretically model the membranes directly. The findings are validated for an irregular cell shape through rigorous numerical modeling.</p></abstract>ARTICLE2014-12-31T00:00:00.000+00:00Skin impedance characterization of bolus material as phantom for use in electrical impedance and computed tomography fusion imaging<abstract><title style='display:none'>Abstract</title><p>Phantoms are widely used in medical imaging to predict image quality prior to clinical imaging. This paper discusses the possible use of bolus material, as a conductivity phantom, for validation and interpretation of electrical impedance tomography (EIT) images. Bolus is commonly used in radiation therapy to mimic tissue. When irradiated, it has radiological characteristics similar to tissue. With increased research interest in CT/EIT fusion imaging there is a need to find a material which has both the absorption coefficient and electrical conductivity similar to biological tissues. In the present study the electrical properties, specifically resistivity, of various commercially available bolus materials were characterized by comparing their frequency response with that of <italic>in-vivo</italic> connective adipose tissue. It was determined that the resistivity of Gelatin Bolus is similar to <italic>in-vivo</italic> tissue in the frequency range 10 kHz to 1MHz and therefore has potential to be used in EIT/CT fusion imaging studies.</p></abstract>ARTICLE2014-04-26T00:00:00.000+00:00Isoconductivity method to study adhesion of yeast cells to gold electrode<abstract><title style='display:none'>Abstract</title><p>In this paper, we used impedance spectroscopy and gold electrodes to detect the presence of yeast cells and monitor the attachment of these cells to the electrodes. We analyzed the effect of conductivity changes of the medium and the attachment on the electrode-electrolyte interface impedance. A three-electrode cell was designed to produce a uniform electric field distribution on the working electrode and to minimize the counter electrode impedance. Moreover, we used a small AC overpotential (10 mV) to keep the system within the linear impedance limits of the electrode-electrolyte interface. This study proposes a new method to differentiate the impedance changes due to the attachment of yeast cells from those due to conductivity changes of the medium. The experiments showed that when the difference between the cell suspension and base solution conductivities is within the experimental error, the impedance changes are only due to the attachment of yeast cells to the electrodes. The experiments also showed a strong dependence (decrease) of the parallel capacity of the electrode electrolyte interface with the yeast cell concentration of suspension. We suggest that this decrease is due to an asymmetrical redistribution of surface charges on both sides of cell, which can be modeled as a biologic capacity connected in series with the double layer capacity of the interface. Our results could help to explain the rate of biofilm formation through the determination of the rate of cell adhesion.</p></abstract>ARTICLE2014-08-21T00:00:00.000+00:00Modelling the Ability of Rheoencephalography to Measure Cerebral Blood Flow<abstract><title style='display:none'>Abstract</title><p>Despite the long history of rheoencephalography (REG), some important aspects of the method are still debatable. Bioimpedance measurements offer great potential benefit for study of the human brain, but the traditional four or six electrode method suffers from potential misinterpretations and lack of accuracy. The objective of this paper is to study the possible mechanism of REG formation by means of numerical modelling using a realistic finite element model of the human head. It is shown that the cardiac related variations in electrical resistivity of the scalp contributes more than 60% to the REG amplitude, whereas the brain and cerebrospinal fluid are mutually compensated by each over.</p></abstract>ARTICLE2014-12-25T00:00:00.000+00:00A short tutorial contribution to impedance and AC-electrokinetic characterization and manipulation of cells and media: Are electric methods more versatile than acoustic and laser methods?<abstract><title style='display:none'>Abstract</title><p>Lab-on-chip systems (LOCs) can be used as <italic>in vitro</italic> systems for cell culture or manipulation in order to analyze or monitor physiological cell parameters. LOCs may combine microfluidic structures with integrated elements such as piezo-transducers, optical tweezers or electrodes for AC-electrokinetic cell and media manipulations. The wide frequency band (&lt;1 kHz to &gt;1 GHz) usable for AC-electrokinetic manipulation and characterization permits avoiding electrochemical electrode processes, undesired cell damage, and provides a choice between different polarization effects that permit a high electric contrast between the cells and the external medium as well as the differentiation between cellular subpopulations according to a variety of parameters. It has been shown that structural polarization effects do not only determine the impedance of cell suspensions and the force effects in AC-electrokinetics but can also be used for the manipulation of media with inhomogeneous temperature distributions. This manuscript considers the interrelations of the impedance of suspensions of cells and AC-electrokinetic single cell effects, such as electroorientation, electrodeformation, dielectrophoresis, electrorotation, and travelling wave (TW) dielectrophoresis. Unified models have allowed us to derive new characteristic equations for the impedance of a suspension of spherical cells, TW dielectrophoresis, and TW pumping. A critical review of the working principles of electro-osmotic, TW and electrothermal micropumps shows the superiority of the electrothermal pumps. Finally, examples are shown for LOC elements that can be produced as metallic structures on glass chips, which may form the bottom plate for self-sealing microfluidic systems. The structures can be used for cell characterization and manipulation but also to realize micropumps or sensors for pH, metabolites, cell-adhesion, etc.</p></abstract>ARTICLE2014-11-02T00:00:00.000+00:00Transient bioimpedance monitoring of mechanotransduction in artificial tissue during indentation<abstract><title style='display:none'>Abstract</title><p>Mechanotransduction is of fundamental importance in cell physiology, facilitating sensing in touch and hearing as well as tissue development and wound healing. This study used an impedance sensor to monitor the effective resistance and permittivity of artificial tissues, alginate hydrogel with encapsulated fibroblasts, which were kept viable through the use of a bespoke microfluidic system. The observed transient impedance responses upon the application of identical compressive normal loads differed between acellular hydrogels and hydrogels in which fibroblasts were encapsulated. These differences resulted from changes in the conductivity and permeability of the hydrogel due to the presence of the encapsulated fibroblasts, and transient changes in ion concentrations due to mechanotransduction effects.</p></abstract>ARTICLE2014-09-09T00:00:00.000+00:00Electrical bioimpedance spectroscopy in time-variant systems: Is undersampling always a problem?<abstract><title style='display:none'>Abstract</title><p>During the last decades, Electrical Bioimpedance Spectroscopy (EBIS) has been applied mainly by using the frequency-sweep technique, across a range of many different applications. Traditionally, the tissue under study is considered to be time-invariant and dynamic changes of tissue activity are ignored by treating the changes as a noise source. A new trend in EBIS is simultaneous electrical stimulation with several frequencies, through the application of a multi-sine, rectangular or other waveform. This method can provide measurements fast enough to sample dynamic changes of different tissues, such as myocard. This high sampling rate comes at a price of reduction in SNR and the increase in complexity of devices. Although the frequency-sweep technique is often inadequate for monitoring the dynamic changes in a variant system, it can be used successfully in applications focused on the time-invariant or slowly-variant part of a system. However, in order to successfully use frequency-sweep EBIS for monitoring time-variant systems, it is paramount to consider the effects of aliasing and especially the folding of higher frequencies, on the desired frequency e.g. DC level. This paper discusses sub-Nyquist sampling of thoracic EBIS measurements and its application in the case of monitoring pulmonary oedema. It is concluded that by considering aliasing, and with proper implementation of smoothing filters, as well as by using random sampling, frequency-sweep EBIS can be used for assessing time-invariant or slowly-variant properties of time-variant biological systems, even in the presence of aliasing. In general, undersampling is not always a problem, but does always require proper consideration.</p></abstract>ARTICLE2014-04-18T00:00:00.000+00:00Geometric parameters optimization of planar interdigitated electrodes for bioimpedance spectroscopy<abstract><title style='display:none'>Abstract</title><p>This paper is concerned with a physical model of an interdigitated sensor working in a frequency range from 100 Hz to 10 MHz. A theoretical approach is proposed to optimize the use of the sensor for bioimpedance spectroscopy. The correlation between design parameters and frequency behavior in coplanar impedance sensors are described. CoventorWare<sup>®</sup> software was used to model the biological medium loaded interdigital sensor in three dimensions to measure its electrical impedance. Complete system simulation by a finite element method (FEM) was used for sensor sensitivity optimization. The influence of geometrical parameters (number of fingers, width of the electrodes) on the impedance spectroscopy of the biological medium was studied. The simulation results are in agreement with the theoretical equations of optimization. Thus, it is possible to design <italic>a priori</italic> such sensor by taking into account the biological medium of interest that will load the sensor.</p></abstract>ARTICLE2013-03-01T00:00:00.000+00:00DC feedback for wide band frequency fixed current source<abstract><title style='display:none'>Abstract</title><p>Alternating current sources are mainly used in bioelectrical impedance devices. Nowadays 50 – 100 kHz bioelectrical impedance devices are commonly used for body composition analysis. High frequency bioelectrical impedance analysis devices are mostly used in bioimpedance tomography and blood analysis. High speed op-amps and voltage comparators are used in this circuit. Direct current feedback is used to prevent delay. An N-Channel J-FET transistor was used to establish the voltage controlled gain amplifier (VCG). A sine wave signal has been applied as input voltage. The value of this signal should be constant in 170 mV rms to keep the output current in about 1 mA rms. Four frequencies; 100 kHz, 1 MHz, 2 MHz and 3.2 MHz were applied to the circuit and the current was measured for different load resistances. The results showed that the current was stable for changes in the resistor load, bouncing around an average point as a result of <italic>bouncing</italic> DC feedback.</p></abstract>ARTICLE2013-03-22T00:00:00.000+00:00Extraction of cardiac and respiration signals in electrical impedance tomography based on independent component analysis<abstract><title style='display:none'>Abstract</title><p>Electrical Impedance Tomography (EIT) has successive wide range in impedance imaging, but still it is difficult to extract cardiac-related conductivity changes and respiratory-related conductivity changes in spontaneous breathing subjects. Quite a few methods are attempted to extract these two signals such as electrocardiogram gated averaging, frequency domain filtering and principal component analysis. However, such methods are not able to take apart these components properly or put some effort in real time imaging and have their own limitations. The purpose of this paper is to introduce a new method in the EIT clinical application field, Independent Component Analysis (ICA) to extract cardiac and respiratory related signals in electrical impedance tomography. Independent component analysis has been introduced to use in electrical impedance tomography but this is the first attempt ever to implement this method to separate these two signals and image those independent conductivity distribution of respiration and cardiac changes independently. Data has been collected from a spontaneous breathing subject. Filtration technique has been used to remove random noise and multi level spatial ICA has been applied to obtain independent component signals which has been later used in reconstruction algorithm for imaging.</p></abstract>ARTICLE2013-10-05T00:00:00.000+00:00Quantitative evaluation of blood glucose concentration using impedance sensing devices<abstract><title style='display:none'>Abstract</title><p>This paper reports on the impedimetric investigation of glucose concentrations present in the human blood by using impedance sensing devices with different working electrode areas. It is evident from the experiment that, the impedance value increases with the increase of glucose concentration in blood and the trend follows in all the devices with various electrode areas. The measured complex admittance plot shows two semicircles which are due to double layer and coating capacitances respectively. Lower values of relative standard deviation in impedance data infer the reproducibility of these devices. A quantitative relationship, developed between the impedance and glucose concentration establishes a positive correlation between the blood glucose values and impedance.</p></abstract>ARTICLE2013-12-31T00:00:00.000+00:00en-us-1