rss_2.0Journal of Electrical Bioimpedance FeedSciendo RSS Feed for Journal of Electrical Bioimpedance of Electrical Bioimpedance 's Cover biotechnical system for screening risk-based diagnosis of COVID-19 and post-COVID syndrome<abstract> <title style='display:none'>Abstract</title> <p>At present, there are no hardware or biochemical systems that allow to assess the severity of post-COVID syndrome in vivo. The hardware of the proposed biotechnical system is based on routine transthoracic electrical impedance rheography, which makes it possible to register the frequency characteristics of the patient's bioimpedance response to controlled stress stimulation, thereby simultaneously fixing the characteristics of his productive heart, the state of the hemomicrocirculatory bed, the efficiency of the gas transport function of his blood, and also reliably assess personal reactivity and adaptive potential. Subsequent mathematical approximation of the obtained biometric data by an original neural network makes it possible to rank the results obtained and automatically generate a program of medical rehabilitation for a particular patient, depending on the severity of his post-COVID syndrome. The study results proved two reliable physiological signs confirming the presence of latent post-COVID complications: a decrease in the base impedance value for light exercise and an increase in the length of the systolic arc of the rheocardiogram.</p> </abstract>ARTICLE2022-09-09T00:00:00.000+00:00Body composition changes in the immediate peri-operative period following total joint arthroplasty<abstract><title style='display:none'>Abstract</title> <sec id="j_joeb-2022-0007_s_005"> <title style='display:none'>Background</title> <p>Bioimpedance analysis (BIA) is a readily available tool to assess body composition in a clinical setting. BIA has received little attention in orthopaedics and namely joint arthroplasty. This study aims to quantify changes in body composition in the immediate peri-operative period following total joint arthroplasty.</p></sec> <sec id="j_joeb-2022-0007_s_006"> <title style='display:none'>Methods</title> <p>Adults scheduled for elective total joint arthroplasty were recruited to participate. Patients underwent BIA scans in the immediate peri-operative period: pre-operative on their day of surgery, post-operative day 0, and post-operative day 1.</p></sec> <sec id="j_joeb-2022-0007_s_007"> <title style='display:none'>Results</title> <p>67 patients were enrolled to undergo BIA scans. Mean age was 62.64 ± 10.28 years old, and 49.2% were females. The all-supine cohort exhibited a 0.36 ± 0.61 kg increase in dry lean mass (<italic>p</italic> &lt; 0.001) and 1.30 ± 2.14 kg increase in lean body mass on postoperative day 0 (<italic>p</italic> &lt; 0.001). Patients received to 1.16 ± 0.58 kg of fluid mass, on average.</p></sec> <sec id="j_joeb-2022-0007_s_008"> <title style='display:none'>Conclusion</title> <p>BIA is a rapid, portable tool that allows for body composition analysis of an inpatient surgical population. This study demonstrated that BIA can detect net fluid changes and may approximate implant mass following total joint arthroplasty. This may aid surgeons in interpreting post-operative body composition changes.</p></sec> </abstract>ARTICLE2022-09-09T00:00:00.000+00:00Bioelectrical impedance changes of the trunk are opposite the limbs following acute hydration change<abstract> <title style='display:none'>Abstract</title> <p>This study aimed to evaluate the changes in impedance and estimates of body composition variables obtained from segmental multi-frequency bioelectrical impedance analysis (SMFBIA) following acute hydration change. All participants (N = 11 active adults) had SMFBIA measurements at baseline (euhydration), post-dehydration, and post-hyperhydration in an experimental repeated-measures design. Dehydration and hyperhydration trials were randomized with the opposite treatment given 24 h later. Dehydration was achieved via a heat chamber of 40 °C and 60% relative humidity. Hyperhydration was achieved by drinking lightly-salted water (30 mmol·L<sup>-1</sup> NaCl; 1.76 g NaCl·L<sup>-1</sup>) within 30 min. Post-measurements were taken 30 min after each treatment. Despite changes in mass post-dehydration (Δ = -2.0%, p &lt; 0.001) and post-hyperhydration (Δ = 1.2%, p &lt; 0.001), SMFBIA estimates of total body water (TBW) did not change significantly across trials (p = 0.507), leading to significant differences (p &lt; 0.001) in SMFBIA-estimates of body fat percentage across trials. Dehydration resulted in a significant (p &lt; 0.001) 8% decrease in limb impedances at both 20 kHz and 100 kHz. Hyperhydration increased limb impedances only slightly (1.5%, p &gt; 0.05). Impedance changes in the trunk followed an opposite pattern of the limbs. SMFBIA failed to track acute changes in TBW. Divergent impedance changes suggest the trunk is influenced by fluid volume, but the limbs are influenced by ion concentration.</p></abstract>ARTICLE2022-06-25T00:00:00.000+00:00Validation of non-empirical fat-free mass estimation model for a wrist-worn device<abstract> <title style='display:none'>Abstract</title> <p>Fat-free mass (FFM) estimation has dramatic importance for body composition evaluation, often providing a basis for treatment of obesity and muscular dystrophy. However, current methods of FFM estimation have several drawbacks, usually related to either cost-effectiveness and equipment size (dual-energy X-ray absorptiometry (DEXA) scan) or model limitations. In this study, we present and validate a new FFM estimation model based on hand-to-hand bioimpedance analysis (BIA) and arm volume. Forty-two participants underwent a full-body DEXA scan, a series of anthropometric measurements, and upper-body BIA measurements with the custom-designed wearable wrist-worn impedance meter. A new two truncated cones (TTC) model was trained on DEXA data and achieved the best performance metrics of 0.886 ± 0.051 r<sup>2</sup>, 0.052 ± 0.009 % mean average error, and 6.884 ± 1.283 kg maximal residual error in FFM estimation. The model further demonstrated its effectiveness in Bland-Altman comparisons with the skinfold thickness-based FFM estimation method, achieving the least mean bias (0.007 kg). The novel TTC model can provide an alternative to full-body BIA measurements, demonstrating an accurate FFM estimation independently of population variables.</p></abstract>ARTICLE2022-06-25T00:00:00.000+00:00Monitoring the skin biophysical parameters among coronavirus patients for three days in a row: a preliminary study<abstract> <title style='display:none'>Abstract</title> <p>The coronavirus epidemic 2019 is spreading all over the world now. Several parameters are used to monitor the status of hospitalized patients; however, monitoring variations in biophysical properties of the skin has not been investigated yet. In this preliminary study, we seek to monitor skin biophysical parameters among coronavirus patients for three days in a row. Skin moisture, pH, sebum, and temperature during the three days were monitored in 30 coronavirus patients by using non-invasive portable instruments. Skin biophysical parameters were increased on the third day of monitoring compared to the first one. In addition, the increase in both skin moisture and temperature were statistically significant. According to the results of this preliminary study, skin biophysical parameters changed (increased) during the specified period in which the patients were monitored. However, changes in skin sebum content and pH were not significant. These skin parameters need to be further investigated until we know their indication ability for the health condition of coronavirus patients in clinical applications.</p></abstract>ARTICLE2022-05-20T00:00:00.000+00:00Influence of acute water ingestion and prolonged standing on raw bioimpedance and subsequent body fluid and composition estimates<abstract> <title style='display:none'>Abstract</title> <p>This study evaluated the influence of acute water ingestion and maintaining an upright posture on raw bioimpedance and subsequent estimates of body fluids and composition. Twenty healthy adults participated in a randomized crossover study. In both conditions, an overnight food and fluid fast was followed by an initial multi-frequency bioimpedance assessment (InBody 770). Participants then ingested 11 mL/kg of water (water condition) or did not (control condition) during a 5-minute period. Thereafter, bioimpedance assessments were performed every 10 minutes for one hour with participants remaining upright throughout. Linear mixed effects models were used to examine the influence of condition and time on raw bioimpedance, body fluids, and body composition. Water consumption increased impedance of the arms but not trunk or legs. However, drift in leg impedance was observed, with decreasing values over time in both conditions. No effects of condition on body fluids were detected, but total body water and intracellular water decreased by ~0.5 kg over time in both conditions. Correspondingly, lean body mass did not differ between conditions but decreased over the measurement duration. The increase in body mass in the water condition was detected exclusively as fat mass, with final fat mass values ~1.3 kg higher than baseline and also higher than the control condition. Acute water ingestion and prolonged standing exert practically meaningful effects on relevant bioimpedance variables quantified by a modern, vertical multi-frequency analyzer. These findings have implications for pre-assessment standardization, methodological reporting, and interpretation of assessments.</p></abstract>ARTICLE2022-05-20T00:00:00.000+00:00Identification of contractions from Electrohysterography for prediction of prolonged labor<abstract><title style='display:none'>Abstract</title><p>The analysis of the uterine electrical activity and its propagation patterns could potentially predict the risk of prolonged/arrested progress of labor. In our study, the Electrohysterography (EHG) signals of 83 participants in labor at around 3-4 cm of cervical dilatation, were recorded for about 30 minutes each. These signals were analyzed for predicting prolonged labor. Out of the 83 participants, 70 participants had normal progress of labor and delivered vaginally. The remaining 13 participants had prolonged/ arrested progress of labor and had to deliver through a cesarean section. In this paper, we propose an algorithm to identify contractions from the acquired EHG signals based on the energy of the signals. The role of contraction consistency and fundal dominance was evaluated for impact on progress of the labor. As per our study, the correlation of contractions was higher in case of normal progress of labor. We also observed that the upper uterine segment was dominant in cases with prolonged/arrested progress of labor.</p></abstract>ARTICLE2022-03-31T00:00:00.000+00:00Opinion: The future of electrical impedance tomography measurement of living with impedance spectroscopy<abstract><title style='display:none'>Abstract</title><p>Impedance spectroscopy is a useful tool for non-invasive and real time measurements of cell suspensions and a variety of biological tissues. The objective of this study was the investigation of the dielectric properties of living aquatic worms (<italic>Lumbriculus variegatus</italic>) using impedance spectroscopy in a frequency range between 100 Hz and 10 MHz. We demonstrate a linear relation between the worm biomass and the phase response of the signal thereby providing a quick and precise method to determine the biomass of aquatic worms <italic>in situ</italic>. Possible applications for non-destructive online biomass monitoring of aquatic worms and other aqueous organisms are discussed. Furthermore, we show that groups of worms fed different diets can be distinguished by the method presented. These results reveal a close relationship between the nutritional composition of the worms and the measured phase response. We also demonstrate that the phase response at 90 kHz does not depend on the worm size. In contrast, the response function for the signal at 440 Hz reveals a linear correlation of average individual worm size and phase. Therefore, we conclude that the measured phase response at 90 kHz qualifies as a measure of the total amount of worm biomass present in the measuring cell, whereas the phase measurement at 440 Hz can be used to estimate the average individual worm size.</p></abstract>ARTICLE2014-12-03T00:00:00.000+00:00Statistical methods for bioimpedance analysis<abstract><title style='display:none'>Abstract</title><p>This paper gives a basic overview of relevant statistical methods for the analysis of bioimpedance measurements, with an aim to answer questions such as: How do I begin with planning an experiment? How many measurements do I need to take? How do I deal with large amounts of frequency sweep data? Which statistical test should I use, and how do I validate my results? Beginning with the hypothesis and the research design, the methodological framework for making inferences based on measurements and statistical analysis is explained. This is followed by a brief discussion on correlated measurements and data reduction before an overview is given of statistical methods for comparison of groups, factor analysis, association, regression and prediction, explained in the context of bioimpedance research. The last chapter is dedicated to the validation of a new method by different measures of performance. A flowchart is presented for selection of statistical method, and a table is given for an overview of the most important terms of performance when evaluating new measurement technology.</p></abstract>ARTICLE2014-04-16T00:00:00.000+00:00The feasibility of using compression bioimpedance measurements to quantify peripheral edema<abstract><title style='display:none'>Abstract</title><p>The accurate assessment of body fluid volume is important in many clinical situations, especially in the determination of “dry weight” in a dialysis setting. Currently, no clinically applicable diagnostic system exists to determine the mechanical properties that accurately characterize peripheral edema in an objective and quantitative manner. We have developed a method for quantifying the impact of compression on the electrical properties of tissue by measuring stress-induced changes in bioimpedance (BIS). Using this method, we simultaneously measured the impedance and mechanical response of a tissue mimicking material (tofu) under both quasi-static and dynamic loading conditions. Our results demonstrate a temporal quantification of viscoelastic properties using a viscoelastic phantom tissue model.</p></abstract>ARTICLE2014-12-25T00:00:00.000+00:00Impedance Ratio Method for Urine Conductivity-Invariant Estimation of Bladder Volume<abstract><title style='display:none'>Abstract</title><p>Non-invasive estimation of bladder volume could help patients with impaired bladder volume sensation to determine the right moment for catheterisation. Continuous, non-invasive impedance measurement is a promising technology in this scenario, although influences of body posture and unknown urine conductivity limit wide clinical use today. We studied impedance changes related to bladder volume by simulation, in-vitro and in-vivo measurements with pigs. In this work, we present a method to reduce the influence of urine conductivity to cystovolumetry and bring bioimpedance cystovolumetry closer to a clinical application.</p></abstract>ARTICLE2014-09-09T00:00:00.000+00:00Simplified estimation of membrane potentials induced by high-frequency electric signals<abstract><title style='display:none'>Abstract</title><p>In this paper we show why the poorly conducting cytoplasmic membranes have little effect on the overall impedance of the tissue above a certain frequency, and derive an estimate of this upper frequency. It is further shown that the induced transmembrane potentials at different sites over the membrane can be found through a simple formula for frequencies above the threshold, without the need to analytically or theoretically model the membranes directly. The findings are validated for an irregular cell shape through rigorous numerical modeling.</p></abstract>ARTICLE2014-12-31T00:00:00.000+00:00Skin impedance characterization of bolus material as phantom for use in electrical impedance and computed tomography fusion imaging<abstract><title style='display:none'>Abstract</title><p>Phantoms are widely used in medical imaging to predict image quality prior to clinical imaging. This paper discusses the possible use of bolus material, as a conductivity phantom, for validation and interpretation of electrical impedance tomography (EIT) images. Bolus is commonly used in radiation therapy to mimic tissue. When irradiated, it has radiological characteristics similar to tissue. With increased research interest in CT/EIT fusion imaging there is a need to find a material which has both the absorption coefficient and electrical conductivity similar to biological tissues. In the present study the electrical properties, specifically resistivity, of various commercially available bolus materials were characterized by comparing their frequency response with that of <italic>in-vivo</italic> connective adipose tissue. It was determined that the resistivity of Gelatin Bolus is similar to <italic>in-vivo</italic> tissue in the frequency range 10 kHz to 1MHz and therefore has potential to be used in EIT/CT fusion imaging studies.</p></abstract>ARTICLE2014-04-26T00:00:00.000+00:00Isoconductivity method to study adhesion of yeast cells to gold electrode<abstract><title style='display:none'>Abstract</title><p>In this paper, we used impedance spectroscopy and gold electrodes to detect the presence of yeast cells and monitor the attachment of these cells to the electrodes. We analyzed the effect of conductivity changes of the medium and the attachment on the electrode-electrolyte interface impedance. A three-electrode cell was designed to produce a uniform electric field distribution on the working electrode and to minimize the counter electrode impedance. Moreover, we used a small AC overpotential (10 mV) to keep the system within the linear impedance limits of the electrode-electrolyte interface. This study proposes a new method to differentiate the impedance changes due to the attachment of yeast cells from those due to conductivity changes of the medium. The experiments showed that when the difference between the cell suspension and base solution conductivities is within the experimental error, the impedance changes are only due to the attachment of yeast cells to the electrodes. The experiments also showed a strong dependence (decrease) of the parallel capacity of the electrode electrolyte interface with the yeast cell concentration of suspension. We suggest that this decrease is due to an asymmetrical redistribution of surface charges on both sides of cell, which can be modeled as a biologic capacity connected in series with the double layer capacity of the interface. Our results could help to explain the rate of biofilm formation through the determination of the rate of cell adhesion.</p></abstract>ARTICLE2014-08-21T00:00:00.000+00:00Modelling the Ability of Rheoencephalography to Measure Cerebral Blood Flow<abstract><title style='display:none'>Abstract</title><p>Despite the long history of rheoencephalography (REG), some important aspects of the method are still debatable. Bioimpedance measurements offer great potential benefit for study of the human brain, but the traditional four or six electrode method suffers from potential misinterpretations and lack of accuracy. The objective of this paper is to study the possible mechanism of REG formation by means of numerical modelling using a realistic finite element model of the human head. It is shown that the cardiac related variations in electrical resistivity of the scalp contributes more than 60% to the REG amplitude, whereas the brain and cerebrospinal fluid are mutually compensated by each over.</p></abstract>ARTICLE2014-12-25T00:00:00.000+00:00A short tutorial contribution to impedance and AC-electrokinetic characterization and manipulation of cells and media: Are electric methods more versatile than acoustic and laser methods?<abstract><title style='display:none'>Abstract</title><p>Lab-on-chip systems (LOCs) can be used as <italic>in vitro</italic> systems for cell culture or manipulation in order to analyze or monitor physiological cell parameters. LOCs may combine microfluidic structures with integrated elements such as piezo-transducers, optical tweezers or electrodes for AC-electrokinetic cell and media manipulations. The wide frequency band (&lt;1 kHz to &gt;1 GHz) usable for AC-electrokinetic manipulation and characterization permits avoiding electrochemical electrode processes, undesired cell damage, and provides a choice between different polarization effects that permit a high electric contrast between the cells and the external medium as well as the differentiation between cellular subpopulations according to a variety of parameters. It has been shown that structural polarization effects do not only determine the impedance of cell suspensions and the force effects in AC-electrokinetics but can also be used for the manipulation of media with inhomogeneous temperature distributions. This manuscript considers the interrelations of the impedance of suspensions of cells and AC-electrokinetic single cell effects, such as electroorientation, electrodeformation, dielectrophoresis, electrorotation, and travelling wave (TW) dielectrophoresis. Unified models have allowed us to derive new characteristic equations for the impedance of a suspension of spherical cells, TW dielectrophoresis, and TW pumping. A critical review of the working principles of electro-osmotic, TW and electrothermal micropumps shows the superiority of the electrothermal pumps. Finally, examples are shown for LOC elements that can be produced as metallic structures on glass chips, which may form the bottom plate for self-sealing microfluidic systems. The structures can be used for cell characterization and manipulation but also to realize micropumps or sensors for pH, metabolites, cell-adhesion, etc.</p></abstract>ARTICLE2014-11-02T00:00:00.000+00:00Transient bioimpedance monitoring of mechanotransduction in artificial tissue during indentation<abstract><title style='display:none'>Abstract</title><p>Mechanotransduction is of fundamental importance in cell physiology, facilitating sensing in touch and hearing as well as tissue development and wound healing. This study used an impedance sensor to monitor the effective resistance and permittivity of artificial tissues, alginate hydrogel with encapsulated fibroblasts, which were kept viable through the use of a bespoke microfluidic system. The observed transient impedance responses upon the application of identical compressive normal loads differed between acellular hydrogels and hydrogels in which fibroblasts were encapsulated. These differences resulted from changes in the conductivity and permeability of the hydrogel due to the presence of the encapsulated fibroblasts, and transient changes in ion concentrations due to mechanotransduction effects.</p></abstract>ARTICLE2014-09-09T00:00:00.000+00:00Electrical bioimpedance spectroscopy in time-variant systems: Is undersampling always a problem?<abstract><title style='display:none'>Abstract</title><p>During the last decades, Electrical Bioimpedance Spectroscopy (EBIS) has been applied mainly by using the frequency-sweep technique, across a range of many different applications. Traditionally, the tissue under study is considered to be time-invariant and dynamic changes of tissue activity are ignored by treating the changes as a noise source. A new trend in EBIS is simultaneous electrical stimulation with several frequencies, through the application of a multi-sine, rectangular or other waveform. This method can provide measurements fast enough to sample dynamic changes of different tissues, such as myocard. This high sampling rate comes at a price of reduction in SNR and the increase in complexity of devices. Although the frequency-sweep technique is often inadequate for monitoring the dynamic changes in a variant system, it can be used successfully in applications focused on the time-invariant or slowly-variant part of a system. However, in order to successfully use frequency-sweep EBIS for monitoring time-variant systems, it is paramount to consider the effects of aliasing and especially the folding of higher frequencies, on the desired frequency e.g. DC level. This paper discusses sub-Nyquist sampling of thoracic EBIS measurements and its application in the case of monitoring pulmonary oedema. It is concluded that by considering aliasing, and with proper implementation of smoothing filters, as well as by using random sampling, frequency-sweep EBIS can be used for assessing time-invariant or slowly-variant properties of time-variant biological systems, even in the presence of aliasing. In general, undersampling is not always a problem, but does always require proper consideration.</p></abstract>ARTICLE2014-04-18T00:00:00.000+00:00en-us-1